A novel functional geranyl diphosphate synthase for scent production in phalaenopsis bellina flowers

Y. Y. Hsiao; W. C. Tsai; H. H. Chen; W. H. Chen

A novel functional geranyl diphosphate synthase for scent production in phalaenopsis bellina flowers

Y. Y. Hsiao, W. C. Tsai, H. H. Chen, W. H. Chen

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Geranyl diphosphate is the precursor for monoterpenes, the major floral scent in Phalaenopsis bellina. The cDNA of P. bellina GDP synthase (PbGDPS) was cloned, and its sequence contained a glutamate-rich motif (Glu-rich) corresponding to the second aspartate-rich motif (SARM) but not any aspartate-rich motifs (Asp-rich). The recombinant PbGDPS enzyme revealed dual prenyltransferase activities by the production of both GDP and farnesyl diphosphate (FDP). Spatial and temporal expression analyses illustrated that the expression of PbGDPS was flower specific and concomitant with maximal emission of monoterpenes on day 5 post-anthesis. In addition, the expression was strongly related to the intensity of the emitted scent among different plant varieties. This is the first report of a functional GDPS without an Asp-rich motif functioning as a homodimer in a plant (P. bellina, Orchidaceae, monocot).

Original language	English
Title of host publication	I International Orchid Symposium
Pages	481-488
Number of pages	8
Publication status	Published - 2010 Oct 29

Publication series

Name	Acta Horticulturae
Volume	878
ISSN (Print)	0567-7572

All Science Journal Classification (ASJC) codes

Horticulture

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Hsiao, Y. Y., Tsai, W. C., Chen, H. H., & Chen, W. H. (2010). A novel functional geranyl diphosphate synthase for scent production in phalaenopsis bellina flowers. In I International Orchid Symposium (pp. 481-488). (Acta Horticulturae; Vol. 878).

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title = "A novel functional geranyl diphosphate synthase for scent production in phalaenopsis bellina flowers",

abstract = "Geranyl diphosphate is the precursor for monoterpenes, the major floral scent in Phalaenopsis bellina. The cDNA of P. bellina GDP synthase (PbGDPS) was cloned, and its sequence contained a glutamate-rich motif (Glu-rich) corresponding to the second aspartate-rich motif (SARM) but not any aspartate-rich motifs (Asp-rich). The recombinant PbGDPS enzyme revealed dual prenyltransferase activities by the production of both GDP and farnesyl diphosphate (FDP). Spatial and temporal expression analyses illustrated that the expression of PbGDPS was flower specific and concomitant with maximal emission of monoterpenes on day 5 post-anthesis. In addition, the expression was strongly related to the intensity of the emitted scent among different plant varieties. This is the first report of a functional GDPS without an Asp-rich motif functioning as a homodimer in a plant (P. bellina, Orchidaceae, monocot).",

author = "Hsiao, {Y. Y.} and Tsai, {W. C.} and Chen, {H. H.} and Chen, {W. H.}",

year = "2010",

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series = "Acta Horticulturae",

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AU - Hsiao, Y. Y.

AU - Tsai, W. C.

AU - Chen, H. H.

AU - Chen, W. H.

PY - 2010/10/29

Y1 - 2010/10/29

N2 - Geranyl diphosphate is the precursor for monoterpenes, the major floral scent in Phalaenopsis bellina. The cDNA of P. bellina GDP synthase (PbGDPS) was cloned, and its sequence contained a glutamate-rich motif (Glu-rich) corresponding to the second aspartate-rich motif (SARM) but not any aspartate-rich motifs (Asp-rich). The recombinant PbGDPS enzyme revealed dual prenyltransferase activities by the production of both GDP and farnesyl diphosphate (FDP). Spatial and temporal expression analyses illustrated that the expression of PbGDPS was flower specific and concomitant with maximal emission of monoterpenes on day 5 post-anthesis. In addition, the expression was strongly related to the intensity of the emitted scent among different plant varieties. This is the first report of a functional GDPS without an Asp-rich motif functioning as a homodimer in a plant (P. bellina, Orchidaceae, monocot).

AB - Geranyl diphosphate is the precursor for monoterpenes, the major floral scent in Phalaenopsis bellina. The cDNA of P. bellina GDP synthase (PbGDPS) was cloned, and its sequence contained a glutamate-rich motif (Glu-rich) corresponding to the second aspartate-rich motif (SARM) but not any aspartate-rich motifs (Asp-rich). The recombinant PbGDPS enzyme revealed dual prenyltransferase activities by the production of both GDP and farnesyl diphosphate (FDP). Spatial and temporal expression analyses illustrated that the expression of PbGDPS was flower specific and concomitant with maximal emission of monoterpenes on day 5 post-anthesis. In addition, the expression was strongly related to the intensity of the emitted scent among different plant varieties. This is the first report of a functional GDPS without an Asp-rich motif functioning as a homodimer in a plant (P. bellina, Orchidaceae, monocot).

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