A novel immunoassay using platinum nanoparticles, silver enhancement and a flatbed scanner

C. H. Yeh; I. L. Wang; H. P. Lin; T. C. Chang; Y. C. Lin

doi:10.1016/j.proche.2009.07.064

A novel immunoassay using platinum nanoparticles, silver enhancement and a flatbed scanner

C. H. Yeh, I. L. Wang, H. P. Lin, T. C. Chang, Y. C. Lin

Research output: Contribution to journal › Conference article › peer-review

5 Citations (Scopus)

Abstract

This study reports a potentially rapid and convenient immunoassay using antibody-platinum nanoparticle (Ab-PtNPs) conjugates as a reporter molecule and a flatbed scanner for detecting immuno-reaction and measuring of the immuno-reaction signal. This study was based on the sandwich immunoassay (three-layer format) which contained a primary antibody, test antigens and a secondary antibody. The results showed that the silver precipitation phenomenon was catalyzed by Ab-PtNPs conjugates. The changing color of reaction could easily be observed by naked eye or scanner. The silver enhancement reaction, a signal amplification method in which silver ions are reduced to silver metal, is introduced to magnify the detection signal. The relationship between sample concentration and detection signal was discussed. And the detection limit (sandwich assay) for the sample antigen was 10^-1 ng/mL. Using a flatbed scanner, Ab-PtNPs conjugates and a silver enhancement reaction, a new immunoassay is constructed.

Original language	English
Pages (from-to)	256-260
Number of pages	5
Journal	Procedia Chemistry
Volume	1
Issue number	1
DOIs	https://doi.org/10.1016/j.proche.2009.07.064
Publication status	Published - 2009 Sept
Event	Eurosensors 23rd Conference - Lausanne, Switzerland Duration: 2009 Sept 6 → 2009 Sept 9

All Science Journal Classification (ASJC) codes

Chemistry(all)
Chemical Engineering(all)

Access to Document

10.1016/j.proche.2009.07.064

Cite this

@article{8233e0e29c4f4fbab2f2eb0bf5d1c913,

title = "A novel immunoassay using platinum nanoparticles, silver enhancement and a flatbed scanner",

abstract = "This study reports a potentially rapid and convenient immunoassay using antibody-platinum nanoparticle (Ab-PtNPs) conjugates as a reporter molecule and a flatbed scanner for detecting immuno-reaction and measuring of the immuno-reaction signal. This study was based on the sandwich immunoassay (three-layer format) which contained a primary antibody, test antigens and a secondary antibody. The results showed that the silver precipitation phenomenon was catalyzed by Ab-PtNPs conjugates. The changing color of reaction could easily be observed by naked eye or scanner. The silver enhancement reaction, a signal amplification method in which silver ions are reduced to silver metal, is introduced to magnify the detection signal. The relationship between sample concentration and detection signal was discussed. And the detection limit (sandwich assay) for the sample antigen was 10-1 ng/mL. Using a flatbed scanner, Ab-PtNPs conjugates and a silver enhancement reaction, a new immunoassay is constructed.",

author = "Yeh, {C. H.} and Wang, {I. L.} and Lin, {H. P.} and Chang, {T. C.} and Lin, {Y. C.}",

year = "2009",

month = sep,

doi = "10.1016/j.proche.2009.07.064",

language = "English",

volume = "1",

pages = "256--260",

journal = "Procedia Chemistry",

issn = "1876-6196",

publisher = "Elsevier BV",

number = "1",

note = "Eurosensors 23rd Conference ; Conference date: 06-09-2009 Through 09-09-2009",

}

TY - JOUR

T1 - A novel immunoassay using platinum nanoparticles, silver enhancement and a flatbed scanner

AU - Yeh, C. H.

AU - Wang, I. L.

AU - Lin, H. P.

AU - Chang, T. C.

AU - Lin, Y. C.

PY - 2009/9

Y1 - 2009/9

N2 - This study reports a potentially rapid and convenient immunoassay using antibody-platinum nanoparticle (Ab-PtNPs) conjugates as a reporter molecule and a flatbed scanner for detecting immuno-reaction and measuring of the immuno-reaction signal. This study was based on the sandwich immunoassay (three-layer format) which contained a primary antibody, test antigens and a secondary antibody. The results showed that the silver precipitation phenomenon was catalyzed by Ab-PtNPs conjugates. The changing color of reaction could easily be observed by naked eye or scanner. The silver enhancement reaction, a signal amplification method in which silver ions are reduced to silver metal, is introduced to magnify the detection signal. The relationship between sample concentration and detection signal was discussed. And the detection limit (sandwich assay) for the sample antigen was 10-1 ng/mL. Using a flatbed scanner, Ab-PtNPs conjugates and a silver enhancement reaction, a new immunoassay is constructed.

AB - This study reports a potentially rapid and convenient immunoassay using antibody-platinum nanoparticle (Ab-PtNPs) conjugates as a reporter molecule and a flatbed scanner for detecting immuno-reaction and measuring of the immuno-reaction signal. This study was based on the sandwich immunoassay (three-layer format) which contained a primary antibody, test antigens and a secondary antibody. The results showed that the silver precipitation phenomenon was catalyzed by Ab-PtNPs conjugates. The changing color of reaction could easily be observed by naked eye or scanner. The silver enhancement reaction, a signal amplification method in which silver ions are reduced to silver metal, is introduced to magnify the detection signal. The relationship between sample concentration and detection signal was discussed. And the detection limit (sandwich assay) for the sample antigen was 10-1 ng/mL. Using a flatbed scanner, Ab-PtNPs conjugates and a silver enhancement reaction, a new immunoassay is constructed.

UR - http://www.scopus.com/inward/record.url?scp=71549161453&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=71549161453&partnerID=8YFLogxK

U2 - 10.1016/j.proche.2009.07.064

DO - 10.1016/j.proche.2009.07.064

M3 - Conference article

AN - SCOPUS:71549161453

SN - 1876-6196

VL - 1

SP - 256

EP - 260

JO - Procedia Chemistry

JF - Procedia Chemistry

IS - 1

T2 - Eurosensors 23rd Conference

Y2 - 6 September 2009 through 9 September 2009

ER -

A novel immunoassay using platinum nanoparticles, silver enhancement and a flatbed scanner

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this