A novel microchip system integrated with gold nano-electrode ensemble for electrochemical determination of hyaluronic acid

Chun Mao Chen, Ming-Long Yeh, Ya Ting Chuang, Che Hsin Lin, Chi Sheng Chien

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This paper proposes an innovative microfluidic device for hyaluronic acid (HA) extraction and electrochemical detection utilizing PMMA based microchip that integrated with gold nanoelectrode ensemble (GNEE). Gold nanoelectrode ensemble is fabricated using electroless deposition in a thin polycarbonate (PC) film and then directly bonded onto the PMMA substrate for high performance electrochemical detection. In the study, microwave is firstly used to decrease the interference of protein on the working electrode, and then electrostatic force is used to separate and extract HA in the rat serum sample. The used of GNEE electrodes in the microchip not only enhances the signal but also decreases the background noises, resulting a high detection limit of 0.1 mg/mL for HA, and showing good linear responses in the concentration range of 10 mg/mL-0.1 mg/mL (R2=0.956). The proposed microchip device provides a fast detection method for hyaluronic acid analysis.

Original languageEnglish
Title of host publicationIEEE Sensors 2009 Conference - SENSORS 2009
Pages1788-1791
Number of pages4
DOIs
Publication statusPublished - 2009 Dec 1
EventIEEE Sensors 2009 Conference - SENSORS 2009 - Christchurch, New Zealand
Duration: 2009 Oct 252009 Oct 28

Publication series

NameProceedings of IEEE Sensors

Other

OtherIEEE Sensors 2009 Conference - SENSORS 2009
CountryNew Zealand
CityChristchurch
Period09-10-2509-10-28

All Science Journal Classification (ASJC) codes

  • Electrical and Electronic Engineering

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