TY - GEN
T1 - A novel microchip system integrated with gold nano-electrode ensemble for electrochemical determination of hyaluronic acid
AU - Chen, Chun Mao
AU - Yeh, Ming-Long
AU - Chuang, Ya Ting
AU - Lin, Che Hsin
AU - Chien, Chi Sheng
PY - 2009/12/1
Y1 - 2009/12/1
N2 - This paper proposes an innovative microfluidic device for hyaluronic acid (HA) extraction and electrochemical detection utilizing PMMA based microchip that integrated with gold nanoelectrode ensemble (GNEE). Gold nanoelectrode ensemble is fabricated using electroless deposition in a thin polycarbonate (PC) film and then directly bonded onto the PMMA substrate for high performance electrochemical detection. In the study, microwave is firstly used to decrease the interference of protein on the working electrode, and then electrostatic force is used to separate and extract HA in the rat serum sample. The used of GNEE electrodes in the microchip not only enhances the signal but also decreases the background noises, resulting a high detection limit of 0.1 mg/mL for HA, and showing good linear responses in the concentration range of 10 mg/mL-0.1 mg/mL (R2=0.956). The proposed microchip device provides a fast detection method for hyaluronic acid analysis.
AB - This paper proposes an innovative microfluidic device for hyaluronic acid (HA) extraction and electrochemical detection utilizing PMMA based microchip that integrated with gold nanoelectrode ensemble (GNEE). Gold nanoelectrode ensemble is fabricated using electroless deposition in a thin polycarbonate (PC) film and then directly bonded onto the PMMA substrate for high performance electrochemical detection. In the study, microwave is firstly used to decrease the interference of protein on the working electrode, and then electrostatic force is used to separate and extract HA in the rat serum sample. The used of GNEE electrodes in the microchip not only enhances the signal but also decreases the background noises, resulting a high detection limit of 0.1 mg/mL for HA, and showing good linear responses in the concentration range of 10 mg/mL-0.1 mg/mL (R2=0.956). The proposed microchip device provides a fast detection method for hyaluronic acid analysis.
UR - http://www.scopus.com/inward/record.url?scp=77951095376&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77951095376&partnerID=8YFLogxK
U2 - 10.1109/ICSENS.2009.5398450
DO - 10.1109/ICSENS.2009.5398450
M3 - Conference contribution
AN - SCOPUS:77951095376
SN - 9781424445486
T3 - Proceedings of IEEE Sensors
SP - 1788
EP - 1791
BT - IEEE Sensors 2009 Conference - SENSORS 2009
T2 - IEEE Sensors 2009 Conference - SENSORS 2009
Y2 - 25 October 2009 through 28 October 2009
ER -