A novel natural tautomeric pair of garcinielliptone FC suppressed nuclear factor κB and induced apoptosis in human colorectal cancer cells

Shen Jeu Won, Ting Yu Lin, Cheng Hsin Yen, Yu Hau Tzeng, Hsiao Sheng Liu, Chun Nan Lin, Cheng Hao Yu, Chi Shiuan Wu, Jyun Ti Chen, Yi Ting Chen, Chi Ying F. Huang, Chun Li Su

Research output: Contribution to journalArticle

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Abstract

The tautomeric pair of garcinielliptone FC (GFC) is a pure and structure-defined active ingredient, isolated from Garcinia subelliptica Merr., a plant with abundant sources of polyphenols. Here, we demonstrate that a higher concentration of GFC had no effect on normal human umbilical vein endothelial cells. On chemoresistant human colorectal cancer HT-29 cells, a time- and dosage-related cytotoxicity was observed. GFC also inhibited the nuclear expression and DNA binding activity of nuclear factor κB in a time- and dosage-related manner using Western blot and electrophoretic mobility shift assay. Furthermore, GFC induced apoptosis, which was characterized by an increase of cells at the sub-G1 phase, induction of DNA fragmentation, and formation of apoptotic bodies using flow cytometry, electrophoresis, and fluorescence microscopy, respectively. Western blot analysis revealed that both caspase-dependent intrinsic and extrinsic pathways as well as caspase-independent pathway were involved. Our data suggest the anticancer mechanisms of GFC on human colorectal cancer.

Original languageEnglish
Pages (from-to)568-578
Number of pages11
JournalJournal of Functional Foods
Volume24
DOIs
Publication statusPublished - 2016 Jun 1

Fingerprint

caspases
colorectal neoplasms
Garcinia subelliptica
Colorectal Neoplasms
Western blotting
apoptosis
Apoptosis
DNA fragmentation
dosage
fluorescence microscopy
active ingredients
interphase
electrophoresis
flow cytometry
cytotoxicity
polyphenols
Caspases
cells
Garcinia
Western Blotting

All Science Journal Classification (ASJC) codes

  • Food Science
  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

Won, Shen Jeu ; Lin, Ting Yu ; Yen, Cheng Hsin ; Tzeng, Yu Hau ; Liu, Hsiao Sheng ; Lin, Chun Nan ; Yu, Cheng Hao ; Wu, Chi Shiuan ; Chen, Jyun Ti ; Chen, Yi Ting ; Huang, Chi Ying F. ; Su, Chun Li. / A novel natural tautomeric pair of garcinielliptone FC suppressed nuclear factor κB and induced apoptosis in human colorectal cancer cells. In: Journal of Functional Foods. 2016 ; Vol. 24. pp. 568-578.
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abstract = "The tautomeric pair of garcinielliptone FC (GFC) is a pure and structure-defined active ingredient, isolated from Garcinia subelliptica Merr., a plant with abundant sources of polyphenols. Here, we demonstrate that a higher concentration of GFC had no effect on normal human umbilical vein endothelial cells. On chemoresistant human colorectal cancer HT-29 cells, a time- and dosage-related cytotoxicity was observed. GFC also inhibited the nuclear expression and DNA binding activity of nuclear factor κB in a time- and dosage-related manner using Western blot and electrophoretic mobility shift assay. Furthermore, GFC induced apoptosis, which was characterized by an increase of cells at the sub-G1 phase, induction of DNA fragmentation, and formation of apoptotic bodies using flow cytometry, electrophoresis, and fluorescence microscopy, respectively. Western blot analysis revealed that both caspase-dependent intrinsic and extrinsic pathways as well as caspase-independent pathway were involved. Our data suggest the anticancer mechanisms of GFC on human colorectal cancer.",
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Won, SJ, Lin, TY, Yen, CH, Tzeng, YH, Liu, HS, Lin, CN, Yu, CH, Wu, CS, Chen, JT, Chen, YT, Huang, CYF & Su, CL 2016, 'A novel natural tautomeric pair of garcinielliptone FC suppressed nuclear factor κB and induced apoptosis in human colorectal cancer cells', Journal of Functional Foods, vol. 24, pp. 568-578. https://doi.org/10.1016/j.jff.2016.05.003

A novel natural tautomeric pair of garcinielliptone FC suppressed nuclear factor κB and induced apoptosis in human colorectal cancer cells. / Won, Shen Jeu; Lin, Ting Yu; Yen, Cheng Hsin; Tzeng, Yu Hau; Liu, Hsiao Sheng; Lin, Chun Nan; Yu, Cheng Hao; Wu, Chi Shiuan; Chen, Jyun Ti; Chen, Yi Ting; Huang, Chi Ying F.; Su, Chun Li.

In: Journal of Functional Foods, Vol. 24, 01.06.2016, p. 568-578.

Research output: Contribution to journalArticle

TY - JOUR

T1 - A novel natural tautomeric pair of garcinielliptone FC suppressed nuclear factor κB and induced apoptosis in human colorectal cancer cells

AU - Won, Shen Jeu

AU - Lin, Ting Yu

AU - Yen, Cheng Hsin

AU - Tzeng, Yu Hau

AU - Liu, Hsiao Sheng

AU - Lin, Chun Nan

AU - Yu, Cheng Hao

AU - Wu, Chi Shiuan

AU - Chen, Jyun Ti

AU - Chen, Yi Ting

AU - Huang, Chi Ying F.

AU - Su, Chun Li

PY - 2016/6/1

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N2 - The tautomeric pair of garcinielliptone FC (GFC) is a pure and structure-defined active ingredient, isolated from Garcinia subelliptica Merr., a plant with abundant sources of polyphenols. Here, we demonstrate that a higher concentration of GFC had no effect on normal human umbilical vein endothelial cells. On chemoresistant human colorectal cancer HT-29 cells, a time- and dosage-related cytotoxicity was observed. GFC also inhibited the nuclear expression and DNA binding activity of nuclear factor κB in a time- and dosage-related manner using Western blot and electrophoretic mobility shift assay. Furthermore, GFC induced apoptosis, which was characterized by an increase of cells at the sub-G1 phase, induction of DNA fragmentation, and formation of apoptotic bodies using flow cytometry, electrophoresis, and fluorescence microscopy, respectively. Western blot analysis revealed that both caspase-dependent intrinsic and extrinsic pathways as well as caspase-independent pathway were involved. Our data suggest the anticancer mechanisms of GFC on human colorectal cancer.

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