A novel ninein-interaction protein, CGI-99, blocks ninein phosphorylation by GSK3β and is highly expressed in brain tumors

Shen Long Howng, Hui Chun Hsu, Tai Shan Cheng, Yun Lin Lee, Li Kwan Chang, Pei-Jung Lu, Yi Ren Hong

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

To explore more hNinein interacting proteins, the yeast two-hybrid screening using ninein C-terminal domain as bait protein was performed. One novel gene, CGI-99, was demonstrated to associate with hNinein in the yeast two-hybrid method and in vitro GST pull-down assay. Molecular characterization also showed that CGI-99 possessed a transcriptional activity at the N-terminal. In addition, CGI-99 formed a dimer with the C-terminal, which overlapped with hNinein binding site. In kinase assay, CGI-99 binds to hNinein and completely blocks the phosphorylation of hNinein by GSK3β. Moreover, CGI-99 was highly expressed in all brain tumors which is in agreement with the Northern blot analysis. Taken together, we have isolated a novel protein CGI-99, which may be involved in the functional regulation of human ninein in the centrosome structure and may also be important in brain development and tumorigenesis.

Original languageEnglish
Pages (from-to)162-168
Number of pages7
JournalFEBS Letters
Volume566
Issue number1-3
DOIs
Publication statusPublished - 2004 May 21

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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