TY - JOUR
T1 - A novel ninein-interaction protein, CGI-99, blocks ninein phosphorylation by GSK3β and is highly expressed in brain tumors
AU - Howng, Shen Long
AU - Hsu, Hui Chun
AU - Cheng, Tai Shan
AU - Lee, Yun Lin
AU - Chang, Li Kwan
AU - Lu, Pei Jung
AU - Hong, Yi Ren
PY - 2004/5/21
Y1 - 2004/5/21
N2 - To explore more hNinein interacting proteins, the yeast two-hybrid screening using ninein C-terminal domain as bait protein was performed. One novel gene, CGI-99, was demonstrated to associate with hNinein in the yeast two-hybrid method and in vitro GST pull-down assay. Molecular characterization also showed that CGI-99 possessed a transcriptional activity at the N-terminal. In addition, CGI-99 formed a dimer with the C-terminal, which overlapped with hNinein binding site. In kinase assay, CGI-99 binds to hNinein and completely blocks the phosphorylation of hNinein by GSK3β. Moreover, CGI-99 was highly expressed in all brain tumors which is in agreement with the Northern blot analysis. Taken together, we have isolated a novel protein CGI-99, which may be involved in the functional regulation of human ninein in the centrosome structure and may also be important in brain development and tumorigenesis.
AB - To explore more hNinein interacting proteins, the yeast two-hybrid screening using ninein C-terminal domain as bait protein was performed. One novel gene, CGI-99, was demonstrated to associate with hNinein in the yeast two-hybrid method and in vitro GST pull-down assay. Molecular characterization also showed that CGI-99 possessed a transcriptional activity at the N-terminal. In addition, CGI-99 formed a dimer with the C-terminal, which overlapped with hNinein binding site. In kinase assay, CGI-99 binds to hNinein and completely blocks the phosphorylation of hNinein by GSK3β. Moreover, CGI-99 was highly expressed in all brain tumors which is in agreement with the Northern blot analysis. Taken together, we have isolated a novel protein CGI-99, which may be involved in the functional regulation of human ninein in the centrosome structure and may also be important in brain development and tumorigenesis.
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U2 - 10.1016/j.febslet.2004.04.024
DO - 10.1016/j.febslet.2004.04.024
M3 - Article
C2 - 15147888
AN - SCOPUS:2442487942
SN - 0014-5793
VL - 566
SP - 162
EP - 168
JO - FEBS Letters
JF - FEBS Letters
IS - 1-3
ER -