A Novel Organophosphorus Pesticide Hydrolase Gene Encoded on a Plasmid in Burkholderia sp. Strain NF100

Kanako Tago, Sumiko Yonezawa, Toshihide Ohkouchi, Tomoyuki Ninomiya, Masayuki Hashimoto, Masahito Hayatsu

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The fenitrothion hydrolase gene encoded on the plasmid pNF2 in Burkholderia sp. Strain NF100 was cloned and sequenced. The 6.5-kbp EcoRI fragment contained three ORFs (ORF1 to 3) the deduced amino acid sequences of which shared no significant homology with any sequence present in databases. The results of frameshift mutation showed that ORF1 and ORF2 were essential for full enzymatic activity, and ORF3 was not involved in the degradation of fenitrothion. Fenitrothion hydrolase was located in the cell membrane fraction. The DNA region containing the fenitrothion hydrolase gene was spontaneously lost from pNF2.

Original languageEnglish
Pages (from-to)53-57
Number of pages5
JournalMicrobes and Environments
Volume21
Issue number1
DOIs
Publication statusPublished - 2006 Jan 1

Fingerprint

fenitrothion
Burkholderia
organophosphorus pesticides
hydrolases
plasmid
plasmids
gene
genes
frameshift mutation
homology
cell membranes
open reading frames
mutation
amino acid sequences
amino acid
membrane
DNA
degradation
organophosphorus pesticide

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Soil Science
  • Plant Science

Cite this

Tago, Kanako ; Yonezawa, Sumiko ; Ohkouchi, Toshihide ; Ninomiya, Tomoyuki ; Hashimoto, Masayuki ; Hayatsu, Masahito. / A Novel Organophosphorus Pesticide Hydrolase Gene Encoded on a Plasmid in Burkholderia sp. Strain NF100. In: Microbes and Environments. 2006 ; Vol. 21, No. 1. pp. 53-57.
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A Novel Organophosphorus Pesticide Hydrolase Gene Encoded on a Plasmid in Burkholderia sp. Strain NF100. / Tago, Kanako; Yonezawa, Sumiko; Ohkouchi, Toshihide; Ninomiya, Tomoyuki; Hashimoto, Masayuki; Hayatsu, Masahito.

In: Microbes and Environments, Vol. 21, No. 1, 01.01.2006, p. 53-57.

Research output: Contribution to journalArticle

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