A Novel Organophosphorus Pesticide Hydrolase Gene Encoded on a Plasmid in Burkholderia sp. Strain NF100

Kanako Tago; Sumiko Yonezawa; Toshihide Ohkouchi; Tomoyuki Ninomiya; Masayuki Hashimoto; Masahito Hayatsu

doi:10.1264/jsme2.21.53

A Novel Organophosphorus Pesticide Hydrolase Gene Encoded on a Plasmid in Burkholderia sp. Strain NF100

Kanako Tago, Sumiko Yonezawa, Toshihide Ohkouchi, Tomoyuki Ninomiya, Masayuki Hashimoto, Masahito Hayatsu

Institute of Molecular Medicine

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

The fenitrothion hydrolase gene encoded on the plasmid pNF2 in Burkholderia sp. Strain NF100 was cloned and sequenced. The 6.5-kbp EcoRI fragment contained three ORFs (ORF1 to 3) the deduced amino acid sequences of which shared no significant homology with any sequence present in databases. The results of frameshift mutation showed that ORF1 and ORF2 were essential for full enzymatic activity, and ORF3 was not involved in the degradation of fenitrothion. Fenitrothion hydrolase was located in the cell membrane fraction. The DNA region containing the fenitrothion hydrolase gene was spontaneously lost from pNF2.

Original language	English
Pages (from-to)	53-57
Number of pages	5
Journal	Microbes and Environments
Volume	21
Issue number	1
DOIs	https://doi.org/10.1264/jsme2.21.53
Publication status	Published - 2006

All Science Journal Classification (ASJC) codes

Ecology, Evolution, Behavior and Systematics
Soil Science
Plant Science

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title = "A Novel Organophosphorus Pesticide Hydrolase Gene Encoded on a Plasmid in Burkholderia sp. Strain NF100",

abstract = "The fenitrothion hydrolase gene encoded on the plasmid pNF2 in Burkholderia sp. Strain NF100 was cloned and sequenced. The 6.5-kbp EcoRI fragment contained three ORFs (ORF1 to 3) the deduced amino acid sequences of which shared no significant homology with any sequence present in databases. The results of frameshift mutation showed that ORF1 and ORF2 were essential for full enzymatic activity, and ORF3 was not involved in the degradation of fenitrothion. Fenitrothion hydrolase was located in the cell membrane fraction. The DNA region containing the fenitrothion hydrolase gene was spontaneously lost from pNF2.",

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T1 - A Novel Organophosphorus Pesticide Hydrolase Gene Encoded on a Plasmid in Burkholderia sp. Strain NF100

AU - Tago, Kanako

AU - Yonezawa, Sumiko

AU - Ohkouchi, Toshihide

AU - Ninomiya, Tomoyuki

AU - Hashimoto, Masayuki

AU - Hayatsu, Masahito

PY - 2006

Y1 - 2006

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AB - The fenitrothion hydrolase gene encoded on the plasmid pNF2 in Burkholderia sp. Strain NF100 was cloned and sequenced. The 6.5-kbp EcoRI fragment contained three ORFs (ORF1 to 3) the deduced amino acid sequences of which shared no significant homology with any sequence present in databases. The results of frameshift mutation showed that ORF1 and ORF2 were essential for full enzymatic activity, and ORF3 was not involved in the degradation of fenitrothion. Fenitrothion hydrolase was located in the cell membrane fraction. The DNA region containing the fenitrothion hydrolase gene was spontaneously lost from pNF2.

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A Novel Organophosphorus Pesticide Hydrolase Gene Encoded on a Plasmid in Burkholderia sp. Strain NF100

Abstract

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