A Novel Organophosphorus Pesticide Hydrolase Gene Encoded on a Plasmid in Burkholderia sp. Strain NF100

Kanako Tago, Sumiko Yonezawa, Toshihide Ohkouchi, Tomoyuki Ninomiya, Masayuki Hashimoto, Masahito Hayatsu

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

The fenitrothion hydrolase gene encoded on the plasmid pNF2 in Burkholderia sp. Strain NF100 was cloned and sequenced. The 6.5-kbp EcoRI fragment contained three ORFs (ORF1 to 3) the deduced amino acid sequences of which shared no significant homology with any sequence present in databases. The results of frameshift mutation showed that ORF1 and ORF2 were essential for full enzymatic activity, and ORF3 was not involved in the degradation of fenitrothion. Fenitrothion hydrolase was located in the cell membrane fraction. The DNA region containing the fenitrothion hydrolase gene was spontaneously lost from pNF2.

Original languageEnglish
Pages (from-to)53-57
Number of pages5
JournalMicrobes and Environments
Volume21
Issue number1
DOIs
Publication statusPublished - 2006

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Soil Science
  • Plant Science

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