TY - JOUR
T1 - A novel polybenzimidazole-modified gold electrode for the analytical determination of hydrogen peroxide
AU - Hua, Mu Yi
AU - Chen, Hsiao Chien
AU - Tsai, Rung Ywan
AU - Lai, Chao Sung
N1 - Funding Information:
We thank the National Science Council and Ministry of Economic Affairs of the Republic of China, and Chang Gung University for financial aid: NSC 100-3113-E-182-001-CC2 , NSC 99-2221-E-182-068 , NSC 98-3114-E-182-001-CC2 , A356AE1120 , and CGURPD280091 .
PY - 2011/7/15
Y1 - 2011/7/15
N2 - The imine of polybenzimidazole (PBI) is chemically oxidized by hydrogen peroxide (H2O2) in the presence of acetic acid (AcOH). Fourier transform infrared (FT-IR) and X-ray photoelectron spectroscopies (XPS) showed that when the AcOH concentration remained constant, the degree of oxidation increased with increasing H2O2 levels. Moreover, the imine also exhibited electrochemical redox behavior. Based on these properties, a PBI-modified Au (PBI/Au) electrode was developed as an enzyme-free H2O2 sensor. At an applied potential of -0.5 V vs. Ag/AgCl, the current response of the PBI/Au electrode was linear with H 2O2 concentration over a range from 0.075 to 1.5 mM, with a sensitivity of 55.0 μA mM-1 cm-2. The probe had excellent stability, with <5% variation from its initial response current after storage at 50 °C for 10 days. Potentially interfering species such as ascorbic or uric acid had no effect on sensitivity. Sensitivity improved dramatically when multiwalled carbon nanotubes (MWCNT) were incorporated in the probe. Under optimal conditions, the detection of H2O2 using a MWCNT-PBI/Au electrode was linear from 1.56 μM to 2.5 mM, with a sensitivity of 928.6 μA mM-1 cm-2. Analysis of H 2O2 concentrations in urine samples using a MWCNT-PBI/Au electrode produced accurate real-time results comparable to those of traditional HPLC methods.
AB - The imine of polybenzimidazole (PBI) is chemically oxidized by hydrogen peroxide (H2O2) in the presence of acetic acid (AcOH). Fourier transform infrared (FT-IR) and X-ray photoelectron spectroscopies (XPS) showed that when the AcOH concentration remained constant, the degree of oxidation increased with increasing H2O2 levels. Moreover, the imine also exhibited electrochemical redox behavior. Based on these properties, a PBI-modified Au (PBI/Au) electrode was developed as an enzyme-free H2O2 sensor. At an applied potential of -0.5 V vs. Ag/AgCl, the current response of the PBI/Au electrode was linear with H 2O2 concentration over a range from 0.075 to 1.5 mM, with a sensitivity of 55.0 μA mM-1 cm-2. The probe had excellent stability, with <5% variation from its initial response current after storage at 50 °C for 10 days. Potentially interfering species such as ascorbic or uric acid had no effect on sensitivity. Sensitivity improved dramatically when multiwalled carbon nanotubes (MWCNT) were incorporated in the probe. Under optimal conditions, the detection of H2O2 using a MWCNT-PBI/Au electrode was linear from 1.56 μM to 2.5 mM, with a sensitivity of 928.6 μA mM-1 cm-2. Analysis of H 2O2 concentrations in urine samples using a MWCNT-PBI/Au electrode produced accurate real-time results comparable to those of traditional HPLC methods.
UR - https://www.scopus.com/pages/publications/79958081594
UR - https://www.scopus.com/pages/publications/79958081594#tab=citedBy
U2 - 10.1016/j.talanta.2011.04.038
DO - 10.1016/j.talanta.2011.04.038
M3 - Article
C2 - 21645751
AN - SCOPUS:79958081594
SN - 0039-9140
VL - 85
SP - 631
EP - 637
JO - Talanta
JF - Talanta
IS - 1
ER -