A novel real-time immunoassay approach utilizing an electro-immunosensing microchip

Min Li, Yu-Cheng Lin, Ka Chu Su, Yu Tsung Wang, Tsung Chain Chang, Hong-Ping Lin

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This study was focused on the development of an Electro-Immunosensing (EIS) microchip for real-time measurement of antibody-antigen recognition in immunoassay. Instead of the enzyme conjugated with secondary antibody for detection, the process of electro-immunoassay was relatively simpler in comparison to the conventional enzyme-linked immunosorbent assay (ELISA). Based on the impedance variety in the frequency domain, we could successfully identify 1 ng/mL protein A with non-specific antigen as the negative control. The EIS chip had higher sensitivity and shorter assay time than the ELISA. Furthermore, the EIS chip could detect the kinematics of antibody-antigen binding.

Original languageEnglish
Title of host publicationTRANSDUCERS '05 - 13th International Conference on Solid-State Sensors and Actuators and Microsystems - Digest of Technical Papers
Pages1620-1623
Number of pages4
Publication statusPublished - 2005 Nov 9
Event13th International Conference on Solid-State Sensors and Actuators and Microsystems, TRANSDUCERS '05 - Seoul, Korea, Republic of
Duration: 2005 Jun 52005 Jun 9

Publication series

NameDigest of Technical Papers - International Conference on Solid State Sensors and Actuators and Microsystems, TRANSDUCERS '05
Volume2

Other

Other13th International Conference on Solid-State Sensors and Actuators and Microsystems, TRANSDUCERS '05
CountryKorea, Republic of
CitySeoul
Period05-06-0505-06-09

Fingerprint

Antigens
Antibodies
Assays
Enzymes
Time measurement
Kinematics
Proteins
Immunosorbents

All Science Journal Classification (ASJC) codes

  • Engineering(all)

Cite this

Li, M., Lin, Y-C., Su, K. C., Wang, Y. T., Chang, T. C., & Lin, H-P. (2005). A novel real-time immunoassay approach utilizing an electro-immunosensing microchip. In TRANSDUCERS '05 - 13th International Conference on Solid-State Sensors and Actuators and Microsystems - Digest of Technical Papers (pp. 1620-1623). [3E4.81] (Digest of Technical Papers - International Conference on Solid State Sensors and Actuators and Microsystems, TRANSDUCERS '05; Vol. 2).
Li, Min ; Lin, Yu-Cheng ; Su, Ka Chu ; Wang, Yu Tsung ; Chang, Tsung Chain ; Lin, Hong-Ping. / A novel real-time immunoassay approach utilizing an electro-immunosensing microchip. TRANSDUCERS '05 - 13th International Conference on Solid-State Sensors and Actuators and Microsystems - Digest of Technical Papers. 2005. pp. 1620-1623 (Digest of Technical Papers - International Conference on Solid State Sensors and Actuators and Microsystems, TRANSDUCERS '05).
@inproceedings{30b93d127b21445183381d7f10133545,
title = "A novel real-time immunoassay approach utilizing an electro-immunosensing microchip",
abstract = "This study was focused on the development of an Electro-Immunosensing (EIS) microchip for real-time measurement of antibody-antigen recognition in immunoassay. Instead of the enzyme conjugated with secondary antibody for detection, the process of electro-immunoassay was relatively simpler in comparison to the conventional enzyme-linked immunosorbent assay (ELISA). Based on the impedance variety in the frequency domain, we could successfully identify 1 ng/mL protein A with non-specific antigen as the negative control. The EIS chip had higher sensitivity and shorter assay time than the ELISA. Furthermore, the EIS chip could detect the kinematics of antibody-antigen binding.",
author = "Min Li and Yu-Cheng Lin and Su, {Ka Chu} and Wang, {Yu Tsung} and Chang, {Tsung Chain} and Hong-Ping Lin",
year = "2005",
month = "11",
day = "9",
language = "English",
isbn = "0780389948",
series = "Digest of Technical Papers - International Conference on Solid State Sensors and Actuators and Microsystems, TRANSDUCERS '05",
pages = "1620--1623",
booktitle = "TRANSDUCERS '05 - 13th International Conference on Solid-State Sensors and Actuators and Microsystems - Digest of Technical Papers",

}

Li, M, Lin, Y-C, Su, KC, Wang, YT, Chang, TC & Lin, H-P 2005, A novel real-time immunoassay approach utilizing an electro-immunosensing microchip. in TRANSDUCERS '05 - 13th International Conference on Solid-State Sensors and Actuators and Microsystems - Digest of Technical Papers., 3E4.81, Digest of Technical Papers - International Conference on Solid State Sensors and Actuators and Microsystems, TRANSDUCERS '05, vol. 2, pp. 1620-1623, 13th International Conference on Solid-State Sensors and Actuators and Microsystems, TRANSDUCERS '05, Seoul, Korea, Republic of, 05-06-05.

A novel real-time immunoassay approach utilizing an electro-immunosensing microchip. / Li, Min; Lin, Yu-Cheng; Su, Ka Chu; Wang, Yu Tsung; Chang, Tsung Chain; Lin, Hong-Ping.

TRANSDUCERS '05 - 13th International Conference on Solid-State Sensors and Actuators and Microsystems - Digest of Technical Papers. 2005. p. 1620-1623 3E4.81 (Digest of Technical Papers - International Conference on Solid State Sensors and Actuators and Microsystems, TRANSDUCERS '05; Vol. 2).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

TY - GEN

T1 - A novel real-time immunoassay approach utilizing an electro-immunosensing microchip

AU - Li, Min

AU - Lin, Yu-Cheng

AU - Su, Ka Chu

AU - Wang, Yu Tsung

AU - Chang, Tsung Chain

AU - Lin, Hong-Ping

PY - 2005/11/9

Y1 - 2005/11/9

N2 - This study was focused on the development of an Electro-Immunosensing (EIS) microchip for real-time measurement of antibody-antigen recognition in immunoassay. Instead of the enzyme conjugated with secondary antibody for detection, the process of electro-immunoassay was relatively simpler in comparison to the conventional enzyme-linked immunosorbent assay (ELISA). Based on the impedance variety in the frequency domain, we could successfully identify 1 ng/mL protein A with non-specific antigen as the negative control. The EIS chip had higher sensitivity and shorter assay time than the ELISA. Furthermore, the EIS chip could detect the kinematics of antibody-antigen binding.

AB - This study was focused on the development of an Electro-Immunosensing (EIS) microchip for real-time measurement of antibody-antigen recognition in immunoassay. Instead of the enzyme conjugated with secondary antibody for detection, the process of electro-immunoassay was relatively simpler in comparison to the conventional enzyme-linked immunosorbent assay (ELISA). Based on the impedance variety in the frequency domain, we could successfully identify 1 ng/mL protein A with non-specific antigen as the negative control. The EIS chip had higher sensitivity and shorter assay time than the ELISA. Furthermore, the EIS chip could detect the kinematics of antibody-antigen binding.

UR - http://www.scopus.com/inward/record.url?scp=27544472866&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=27544472866&partnerID=8YFLogxK

M3 - Conference contribution

SN - 0780389948

SN - 9780780389946

T3 - Digest of Technical Papers - International Conference on Solid State Sensors and Actuators and Microsystems, TRANSDUCERS '05

SP - 1620

EP - 1623

BT - TRANSDUCERS '05 - 13th International Conference on Solid-State Sensors and Actuators and Microsystems - Digest of Technical Papers

ER -

Li M, Lin Y-C, Su KC, Wang YT, Chang TC, Lin H-P. A novel real-time immunoassay approach utilizing an electro-immunosensing microchip. In TRANSDUCERS '05 - 13th International Conference on Solid-State Sensors and Actuators and Microsystems - Digest of Technical Papers. 2005. p. 1620-1623. 3E4.81. (Digest of Technical Papers - International Conference on Solid State Sensors and Actuators and Microsystems, TRANSDUCERS '05).