A novel real-time immunoassay approach utilizing an electro-immunosensing microchip

Min Li, Yu Cheng Lin, Ka Chu Su, Yu Tsung Wang, Tsung Chain Chang, Hong Ping Lin

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This study was focused on the development of an Electro-Immunosensing (EIS) microchip for real-time measurement of antibody-antigen recognition in immunoassay. Instead of the enzyme conjugated with secondary antibody for detection, the process of electro-immunoassay was relatively simpler in comparison to the conventional enzyme-linked immunosorbent assay (ELISA). Based on the impedance variety in the frequency domain, we could successfully identify 1 ng/mL protein A with non-specific antigen as the negative control. The EIS chip had higher sensitivity and shorter assay time than the ELISA. Furthermore, the EIS chip could detect the kinematics of antibody-antigen binding.

Original languageEnglish
Title of host publicationTRANSDUCERS '05 - 13th International Conference on Solid-State Sensors and Actuators and Microsystems - Digest of Technical Papers
Pages1620-1623
Number of pages4
Publication statusPublished - 2005
Event13th International Conference on Solid-State Sensors and Actuators and Microsystems, TRANSDUCERS '05 - Seoul, Korea, Republic of
Duration: 2005 Jun 52005 Jun 9

Publication series

NameDigest of Technical Papers - International Conference on Solid State Sensors and Actuators and Microsystems, TRANSDUCERS '05
Volume2

Other

Other13th International Conference on Solid-State Sensors and Actuators and Microsystems, TRANSDUCERS '05
Country/TerritoryKorea, Republic of
CitySeoul
Period05-06-0505-06-09

All Science Journal Classification (ASJC) codes

  • General Engineering

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