A novel real-time immunoassay utilizing an electro-immunosensing microchip and gold nanoparticles for signal enhancement

Min Li; Yu Cheng Lin; Kai Chun Su; Yu Tsung Wang; Tsung Chain Chang; Hong Ping Lin

doi:10.1016/j.snb.2005.12.055

A novel real-time immunoassay utilizing an electro-immunosensing microchip and gold nanoparticles for signal enhancement

Min Li, Yu Cheng Lin, Kai Chun Su, Yu Tsung Wang, Tsung Chain Chang, Hong Ping Lin

Research output: Contribution to journal › Article › peer-review

26 Citations (Scopus)

Abstract

This study focused on the development of an electro-immunosensing (EIS) microchip for real-time measurement of antibody-antigen recognition in immunoassay. Instead of the enzyme being conjugated with a secondary antibody for detection, the process of electro-immunoassay was found to be relatively simpler compared to the conventional enzyme-linked immunosorbent assay (ELISA). This novel testing method was designed using the wave impedance theory to measure the antibody-antigen recognition. Based on the phase variety in the frequency domain, the detection limit of protein A was 1 ng/mL. It was found that the antibody-antigen recognition has an obvious peak of the phase angle near 1.61 GHz. The EIS chip had higher sensitivity and a shorter assay time than the ELISA. The sensitivity of the immunoassay on the EIS chip was 100-fold higher than that of conventional enzyme-linked immunosorbent assay (ELISA). Using antibodies labelled with 13 nm gold nanoparticles (ANPs), the detection sensitivity of protein A was increased to 0.1 ng/mL. In addition, the EIS chip was capable of detecting the kinematics of antibody-antigen binding.

Original language	English
Pages (from-to)	451-456
Number of pages	6
Journal	Sensors and Actuators, B: Chemical
Volume	117
Issue number	2
DOIs	https://doi.org/10.1016/j.snb.2005.12.055
Publication status	Published - 2006 Oct 12

All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
Instrumentation
Condensed Matter Physics
Surfaces, Coatings and Films
Metals and Alloys
Electrical and Electronic Engineering
Materials Chemistry

Access to Document

10.1016/j.snb.2005.12.055

Cite this

@article{f893e8a738664f8b821b778f468b9af0,

title = "A novel real-time immunoassay utilizing an electro-immunosensing microchip and gold nanoparticles for signal enhancement",

abstract = "This study focused on the development of an electro-immunosensing (EIS) microchip for real-time measurement of antibody-antigen recognition in immunoassay. Instead of the enzyme being conjugated with a secondary antibody for detection, the process of electro-immunoassay was found to be relatively simpler compared to the conventional enzyme-linked immunosorbent assay (ELISA). This novel testing method was designed using the wave impedance theory to measure the antibody-antigen recognition. Based on the phase variety in the frequency domain, the detection limit of protein A was 1 ng/mL. It was found that the antibody-antigen recognition has an obvious peak of the phase angle near 1.61 GHz. The EIS chip had higher sensitivity and a shorter assay time than the ELISA. The sensitivity of the immunoassay on the EIS chip was 100-fold higher than that of conventional enzyme-linked immunosorbent assay (ELISA). Using antibodies labelled with 13 nm gold nanoparticles (ANPs), the detection sensitivity of protein A was increased to 0.1 ng/mL. In addition, the EIS chip was capable of detecting the kinematics of antibody-antigen binding.",

author = "Min Li and Lin, {Yu Cheng} and Su, {Kai Chun} and Wang, {Yu Tsung} and Chang, {Tsung Chain} and Lin, {Hong Ping}",

note = "Funding Information: The authors would like to thank the Center for Micro/Nano Technology Research, National Cheng Kung University, Tainan, Taiwan, ROC for access to their equipment and for their technical support. Funding from the Ministry of Education and the National Science Council of Taiwan, ROC is gratefully acknowledged (NSC94-2323-B-006-005).",

year = "2006",

month = oct,

day = "12",

doi = "10.1016/j.snb.2005.12.055",

language = "English",

volume = "117",

pages = "451--456",

journal = "Sensors and Actuators, B: Chemical",

issn = "0925-4005",

publisher = "Elsevier BV",

number = "2",

}

TY - JOUR

T1 - A novel real-time immunoassay utilizing an electro-immunosensing microchip and gold nanoparticles for signal enhancement

AU - Li, Min

AU - Lin, Yu Cheng

AU - Su, Kai Chun

AU - Wang, Yu Tsung

AU - Chang, Tsung Chain

AU - Lin, Hong Ping

N1 - Funding Information: The authors would like to thank the Center for Micro/Nano Technology Research, National Cheng Kung University, Tainan, Taiwan, ROC for access to their equipment and for their technical support. Funding from the Ministry of Education and the National Science Council of Taiwan, ROC is gratefully acknowledged (NSC94-2323-B-006-005).

PY - 2006/10/12

Y1 - 2006/10/12

N2 - This study focused on the development of an electro-immunosensing (EIS) microchip for real-time measurement of antibody-antigen recognition in immunoassay. Instead of the enzyme being conjugated with a secondary antibody for detection, the process of electro-immunoassay was found to be relatively simpler compared to the conventional enzyme-linked immunosorbent assay (ELISA). This novel testing method was designed using the wave impedance theory to measure the antibody-antigen recognition. Based on the phase variety in the frequency domain, the detection limit of protein A was 1 ng/mL. It was found that the antibody-antigen recognition has an obvious peak of the phase angle near 1.61 GHz. The EIS chip had higher sensitivity and a shorter assay time than the ELISA. The sensitivity of the immunoassay on the EIS chip was 100-fold higher than that of conventional enzyme-linked immunosorbent assay (ELISA). Using antibodies labelled with 13 nm gold nanoparticles (ANPs), the detection sensitivity of protein A was increased to 0.1 ng/mL. In addition, the EIS chip was capable of detecting the kinematics of antibody-antigen binding.

AB - This study focused on the development of an electro-immunosensing (EIS) microchip for real-time measurement of antibody-antigen recognition in immunoassay. Instead of the enzyme being conjugated with a secondary antibody for detection, the process of electro-immunoassay was found to be relatively simpler compared to the conventional enzyme-linked immunosorbent assay (ELISA). This novel testing method was designed using the wave impedance theory to measure the antibody-antigen recognition. Based on the phase variety in the frequency domain, the detection limit of protein A was 1 ng/mL. It was found that the antibody-antigen recognition has an obvious peak of the phase angle near 1.61 GHz. The EIS chip had higher sensitivity and a shorter assay time than the ELISA. The sensitivity of the immunoassay on the EIS chip was 100-fold higher than that of conventional enzyme-linked immunosorbent assay (ELISA). Using antibodies labelled with 13 nm gold nanoparticles (ANPs), the detection sensitivity of protein A was increased to 0.1 ng/mL. In addition, the EIS chip was capable of detecting the kinematics of antibody-antigen binding.

UR - http://www.scopus.com/inward/record.url?scp=33748059896&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33748059896&partnerID=8YFLogxK

U2 - 10.1016/j.snb.2005.12.055

DO - 10.1016/j.snb.2005.12.055

M3 - Article

AN - SCOPUS:33748059896

SN - 0925-4005

VL - 117

SP - 451

EP - 456

JO - Sensors and Actuators, B: Chemical

JF - Sensors and Actuators, B: Chemical

IS - 2

ER -

A novel real-time immunoassay utilizing an electro-immunosensing microchip and gold nanoparticles for signal enhancement

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this