TY - JOUR
T1 - A novel real-time immunoassay utilizing an electro-immunosensing microchip and gold nanoparticles for signal enhancement
AU - Li, Min
AU - Lin, Yu Cheng
AU - Su, Kai Chun
AU - Wang, Yu Tsung
AU - Chang, Tsung Chain
AU - Lin, Hong Ping
N1 - Funding Information:
The authors would like to thank the Center for Micro/Nano Technology Research, National Cheng Kung University, Tainan, Taiwan, ROC for access to their equipment and for their technical support. Funding from the Ministry of Education and the National Science Council of Taiwan, ROC is gratefully acknowledged (NSC94-2323-B-006-005).
Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2006/10/12
Y1 - 2006/10/12
N2 - This study focused on the development of an electro-immunosensing (EIS) microchip for real-time measurement of antibody-antigen recognition in immunoassay. Instead of the enzyme being conjugated with a secondary antibody for detection, the process of electro-immunoassay was found to be relatively simpler compared to the conventional enzyme-linked immunosorbent assay (ELISA). This novel testing method was designed using the wave impedance theory to measure the antibody-antigen recognition. Based on the phase variety in the frequency domain, the detection limit of protein A was 1 ng/mL. It was found that the antibody-antigen recognition has an obvious peak of the phase angle near 1.61 GHz. The EIS chip had higher sensitivity and a shorter assay time than the ELISA. The sensitivity of the immunoassay on the EIS chip was 100-fold higher than that of conventional enzyme-linked immunosorbent assay (ELISA). Using antibodies labelled with 13 nm gold nanoparticles (ANPs), the detection sensitivity of protein A was increased to 0.1 ng/mL. In addition, the EIS chip was capable of detecting the kinematics of antibody-antigen binding.
AB - This study focused on the development of an electro-immunosensing (EIS) microchip for real-time measurement of antibody-antigen recognition in immunoassay. Instead of the enzyme being conjugated with a secondary antibody for detection, the process of electro-immunoassay was found to be relatively simpler compared to the conventional enzyme-linked immunosorbent assay (ELISA). This novel testing method was designed using the wave impedance theory to measure the antibody-antigen recognition. Based on the phase variety in the frequency domain, the detection limit of protein A was 1 ng/mL. It was found that the antibody-antigen recognition has an obvious peak of the phase angle near 1.61 GHz. The EIS chip had higher sensitivity and a shorter assay time than the ELISA. The sensitivity of the immunoassay on the EIS chip was 100-fold higher than that of conventional enzyme-linked immunosorbent assay (ELISA). Using antibodies labelled with 13 nm gold nanoparticles (ANPs), the detection sensitivity of protein A was increased to 0.1 ng/mL. In addition, the EIS chip was capable of detecting the kinematics of antibody-antigen binding.
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U2 - 10.1016/j.snb.2005.12.055
DO - 10.1016/j.snb.2005.12.055
M3 - Article
AN - SCOPUS:33748059896
VL - 117
SP - 451
EP - 456
JO - Sensors and Actuators, B: Chemical
JF - Sensors and Actuators, B: Chemical
SN - 0925-4005
IS - 2
ER -