A novel real-time immunoassay utilizing an electro-immunosensing microchip and gold nanoparticles for signal enhancement

Min Li, Yu Cheng Lin, Kai Chun Su, Yu Tsung Wang, Tsung Chain Chang, Hong Ping Lin

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

This study focused on the development of an electro-immunosensing (EIS) microchip for real-time measurement of antibody-antigen recognition in immunoassay. Instead of the enzyme being conjugated with a secondary antibody for detection, the process of electro-immunoassay was found to be relatively simpler compared to the conventional enzyme-linked immunosorbent assay (ELISA). This novel testing method was designed using the wave impedance theory to measure the antibody-antigen recognition. Based on the phase variety in the frequency domain, the detection limit of protein A was 1 ng/mL. It was found that the antibody-antigen recognition has an obvious peak of the phase angle near 1.61 GHz. The EIS chip had higher sensitivity and a shorter assay time than the ELISA. The sensitivity of the immunoassay on the EIS chip was 100-fold higher than that of conventional enzyme-linked immunosorbent assay (ELISA). Using antibodies labelled with 13 nm gold nanoparticles (ANPs), the detection sensitivity of protein A was increased to 0.1 ng/mL. In addition, the EIS chip was capable of detecting the kinematics of antibody-antigen binding.

Original languageEnglish
Pages (from-to)451-456
Number of pages6
JournalSensors and Actuators, B: Chemical
Volume117
Issue number2
DOIs
Publication statusPublished - 2006 Oct 12

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

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