A novel ultrahigh-resolution surface plasmon resonance biosensor with an Au nanocluster-embedded dielectric film

W. P. Hu, S. J. Chen, K. T. Huang, J. H. Hsu, W. Y. Chen, G. L. Chang, K. A. Lai

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

The detection performance of conventional surface plasmon resonance (SPR) biosensors is limited to a 1pg/mm2 surface coverage of biomolecules, and consequently, such sensors struggle to detect the interaction of small molecules in low concentrations. The present study is attempted to propose the use of a novel SPR biosensor with Au nanoclusters embedded in a dielectric film to achieve a 10-fold improvement in the resolution performance. A co-sputtering method utilizing a multi-target sputtering system is used to fabricate the present dielectric films (SiO2) with embedded Au nanoclusters. It is shown that the sensitivity of the developed SPR biosensor can be improved by adjusting the size and volume fraction of the embedded Au nanoclusters in order to control the surface plasmon effect. The present gas detection and DNA hybridization experimental results confirm that the proposed Au nanocluster-enhanced SPR biosensor provides the potential to achieve an ultrahigh-resolution detection performance of approximately 0.1pg/mm 2 surface coverage of biomolecules.

Original languageEnglish
Pages (from-to)1465-1471
Number of pages7
JournalBiosensors and Bioelectronics
Volume19
Issue number11
DOIs
Publication statusPublished - 2004 Jun 15

Fingerprint

Dielectric films
Surface Plasmon Resonance
Nanoclusters
Surface plasmon resonance
Biosensing Techniques
Biosensors
Biomolecules
Sputtering
Volume fraction
DNA
Gases
Molecules
Sensors

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

Cite this

Hu, W. P. ; Chen, S. J. ; Huang, K. T. ; Hsu, J. H. ; Chen, W. Y. ; Chang, G. L. ; Lai, K. A. / A novel ultrahigh-resolution surface plasmon resonance biosensor with an Au nanocluster-embedded dielectric film. In: Biosensors and Bioelectronics. 2004 ; Vol. 19, No. 11. pp. 1465-1471.
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A novel ultrahigh-resolution surface plasmon resonance biosensor with an Au nanocluster-embedded dielectric film. / Hu, W. P.; Chen, S. J.; Huang, K. T.; Hsu, J. H.; Chen, W. Y.; Chang, G. L.; Lai, K. A.

In: Biosensors and Bioelectronics, Vol. 19, No. 11, 15.06.2004, p. 1465-1471.

Research output: Contribution to journalArticle

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