TY - JOUR
T1 - A Perina nuda cell line (NTU-Pn-HF) from pupal ovary that is persistently infected with a picorna-like virus (PnPV)
AU - Wu, Chih Yu
AU - Yang, Hsi Nan
AU - Lo, Chu Fang
AU - Wang, Chung Hsiung
PY - 2002/2
Y1 - 2002/2
N2 - A new cell line, designated NTU-Pn-HF, was established from the pupal ovary of Perina nuda Fabricius (Lepidoptera: Lymantriidae) and characterized by distinct morphological properties and molecular markers. Pn-HF cells are highly susceptible to P. nuda nucleopolyhedrovirus (PenuNPV). Surprisingly, some of the sub-cultural Pn-HF cells suffered an obvious cytopathic effect (CPE) and proceeded to die, while most of the cells remained healthy with good nutrition. Under electron microscopy, most of the Pn-HF cells were found to be carriers of a picorna-like virus. A specific anti-P. nuda picorna-like virus (PnPV) antiserum and RT-PCR with a primer set designed from the sequence of a putative PnPV's helicase gene confirmed that the picorna-like virus in Pn-HF cells is PnPV (Wang et al., 1999, J. Invertebr. Pathol. 74: 62-68), and Pn-HF cells are persistently infected with PnPV. This finding implies that the Pn-HF cell line can be a PnPV supporter for further work, and also a convenient tool in studying the pathogenesis of insect picornavirus and the mechanism of the picornavirus-persistent infection.
AB - A new cell line, designated NTU-Pn-HF, was established from the pupal ovary of Perina nuda Fabricius (Lepidoptera: Lymantriidae) and characterized by distinct morphological properties and molecular markers. Pn-HF cells are highly susceptible to P. nuda nucleopolyhedrovirus (PenuNPV). Surprisingly, some of the sub-cultural Pn-HF cells suffered an obvious cytopathic effect (CPE) and proceeded to die, while most of the cells remained healthy with good nutrition. Under electron microscopy, most of the Pn-HF cells were found to be carriers of a picorna-like virus. A specific anti-P. nuda picorna-like virus (PnPV) antiserum and RT-PCR with a primer set designed from the sequence of a putative PnPV's helicase gene confirmed that the picorna-like virus in Pn-HF cells is PnPV (Wang et al., 1999, J. Invertebr. Pathol. 74: 62-68), and Pn-HF cells are persistently infected with PnPV. This finding implies that the Pn-HF cell line can be a PnPV supporter for further work, and also a convenient tool in studying the pathogenesis of insect picornavirus and the mechanism of the picornavirus-persistent infection.
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U2 - 10.1303/aez.2002.171
DO - 10.1303/aez.2002.171
M3 - Article
AN - SCOPUS:0036488609
SN - 0003-6862
VL - 37
SP - 171
EP - 179
JO - Applied Entomology and Zoology
JF - Applied Entomology and Zoology
IS - 1
ER -