A plant type 2 metallothionein (MT) from cork tissue responds to oxidative stress

Gisela Mir, Jordi Domènech, Gemma Huguet, Woei-Jiun Guo, Peter Goldsbrough, Silvia Atrian, Marisa Molinas

Research output: Contribution to journalArticle

142 Citations (Scopus)

Abstract

Expression of plant metallothionein genes has been reported in a variety of senescing tissues, such as leaves and stems, ripening fruits, and wounded tissues, and has been proposed to function in both metal chaperoning and scavenging of reactive oxygen species. In this work, it is shown that MT is also associated with suberization, after identifying a gene actively transcribed in Quercus suber cork cells as a novel MT. This cDNA, isolated from a phellem cDNA library, encodes a MT that belongs to type 2 plant MTs (QsMT). Expression of the QsMT cDNA in E. coli grown in media supplemented with Zn, Cd, or Cu has yielded recombinant QsMT. Characterization of the respective metal aggregates agrees well with a copper-related biological role, consistent with the capacity of QsMT to restore copper tolerance to a MT-deficient, copper-sensitive yeast mutant. Furthermore, in situ hybridization results demonstrate that RNA expression of QsMT is mainly observed under conditions related to oxidative stress, either endogenous, as found in cork or in actively proliferating tissues, or exogenous, for example, in response to H2O2 or paraquat treatments. The putative role of QsMT in oxidative stress, both as a free radical scavenger via its sulphydryl groups or as a copper chelator is discussed.

Original languageEnglish
Pages (from-to)2483-2493
Number of pages11
JournalJournal of Experimental Botany
Volume55
Issue number408
DOIs
Publication statusPublished - 2004 Dec 1

Fingerprint

cork
Metallothionein
metallothionein
Oxidative Stress
oxidative stress
Copper
copper
Complementary DNA
Metals
Quercus
Plant Genes
Free Radical Scavengers
Paraquat
metals
suberization
Chelating Agents
Gene Library
periderm
Quercus suber
paraquat

All Science Journal Classification (ASJC) codes

  • Physiology
  • Plant Science

Cite this

Mir, G., Domènech, J., Huguet, G., Guo, W-J., Goldsbrough, P., Atrian, S., & Molinas, M. (2004). A plant type 2 metallothionein (MT) from cork tissue responds to oxidative stress. Journal of Experimental Botany, 55(408), 2483-2493. https://doi.org/10.1093/jxb/erh254
Mir, Gisela ; Domènech, Jordi ; Huguet, Gemma ; Guo, Woei-Jiun ; Goldsbrough, Peter ; Atrian, Silvia ; Molinas, Marisa. / A plant type 2 metallothionein (MT) from cork tissue responds to oxidative stress. In: Journal of Experimental Botany. 2004 ; Vol. 55, No. 408. pp. 2483-2493.
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abstract = "Expression of plant metallothionein genes has been reported in a variety of senescing tissues, such as leaves and stems, ripening fruits, and wounded tissues, and has been proposed to function in both metal chaperoning and scavenging of reactive oxygen species. In this work, it is shown that MT is also associated with suberization, after identifying a gene actively transcribed in Quercus suber cork cells as a novel MT. This cDNA, isolated from a phellem cDNA library, encodes a MT that belongs to type 2 plant MTs (QsMT). Expression of the QsMT cDNA in E. coli grown in media supplemented with Zn, Cd, or Cu has yielded recombinant QsMT. Characterization of the respective metal aggregates agrees well with a copper-related biological role, consistent with the capacity of QsMT to restore copper tolerance to a MT-deficient, copper-sensitive yeast mutant. Furthermore, in situ hybridization results demonstrate that RNA expression of QsMT is mainly observed under conditions related to oxidative stress, either endogenous, as found in cork or in actively proliferating tissues, or exogenous, for example, in response to H2O2 or paraquat treatments. The putative role of QsMT in oxidative stress, both as a free radical scavenger via its sulphydryl groups or as a copper chelator is discussed.",
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Mir, G, Domènech, J, Huguet, G, Guo, W-J, Goldsbrough, P, Atrian, S & Molinas, M 2004, 'A plant type 2 metallothionein (MT) from cork tissue responds to oxidative stress', Journal of Experimental Botany, vol. 55, no. 408, pp. 2483-2493. https://doi.org/10.1093/jxb/erh254

A plant type 2 metallothionein (MT) from cork tissue responds to oxidative stress. / Mir, Gisela; Domènech, Jordi; Huguet, Gemma; Guo, Woei-Jiun; Goldsbrough, Peter; Atrian, Silvia; Molinas, Marisa.

In: Journal of Experimental Botany, Vol. 55, No. 408, 01.12.2004, p. 2483-2493.

Research output: Contribution to journalArticle

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T1 - A plant type 2 metallothionein (MT) from cork tissue responds to oxidative stress

AU - Mir, Gisela

AU - Domènech, Jordi

AU - Huguet, Gemma

AU - Guo, Woei-Jiun

AU - Goldsbrough, Peter

AU - Atrian, Silvia

AU - Molinas, Marisa

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