TY - JOUR
T1 - A rapid paper-based detection system for determination of human serum albumin concentration
AU - Yang, Ruey Jen
AU - Tseng, Chin Chung
AU - Ju, Wei Jhong
AU - Wang, Hsiang Li
AU - Fu, Lung Ming
N1 - Funding Information:
This study was supported by the Ministry of Science and Technology of Taiwan ( 106-2221-E-020-019-MY3 and 106-2314-B-020-002-MY3 ).
PY - 2018/11/15
Y1 - 2018/11/15
N2 - A rapid analytical platform composed of a paper-based chip (µPB-Chip) and a small analytic box is developed to determine the concentration of human serum albumin (HSA). In this study, a Bromocresol Green (BCG) reagent is dropped into the reaction area of the µPB-Chip, and the HSA concentration was derived from the R (red) value intensity of the albumin-BCG complex formed after a reaction process at 37 °C for 12 min. The effectiveness of the developed method is displayed using albumin concentrations ranging from 0 to 5 g/dL in HSA control samples. The measurement results acquired from 36 volunteer patient's HSA samples are presented to be in good consistency with those obtained utilizing a conventional assay technique (R2 = 0.9912). Ultimately, the results show that the developed platform provides a convenient, economical and dependable method for HSA concentration detection.
AB - A rapid analytical platform composed of a paper-based chip (µPB-Chip) and a small analytic box is developed to determine the concentration of human serum albumin (HSA). In this study, a Bromocresol Green (BCG) reagent is dropped into the reaction area of the µPB-Chip, and the HSA concentration was derived from the R (red) value intensity of the albumin-BCG complex formed after a reaction process at 37 °C for 12 min. The effectiveness of the developed method is displayed using albumin concentrations ranging from 0 to 5 g/dL in HSA control samples. The measurement results acquired from 36 volunteer patient's HSA samples are presented to be in good consistency with those obtained utilizing a conventional assay technique (R2 = 0.9912). Ultimately, the results show that the developed platform provides a convenient, economical and dependable method for HSA concentration detection.
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U2 - 10.1016/j.cej.2018.07.022
DO - 10.1016/j.cej.2018.07.022
M3 - Article
AN - SCOPUS:85049438304
VL - 352
SP - 241
EP - 246
JO - Chemical Engineering Journal
JF - Chemical Engineering Journal
SN - 1385-8947
ER -