Abstract
Anoectochilus formosanus is a famous traditional Taiwanese folk medicine. Kinsenoside (1), one of the major active components in A. formosanus, exhibits hepatoprotective, antihyperliposis and antiflammatory activities. In this study, a proton nuclear magnetic resonance (1H NMR) method was developed for rapid quantitative analysis of several bioactive major constituents, including kinsenoside (1), 3-(R)-3-β-d-gluco-pyranosyloxy-4-hydroxybutanoic acid (2), 1-O-isopropyl-β-d-gluco-pyranoside (3), 4-(β-d-gluco-pyranosyloxy)benzyl alcohol (4) and benzoic acid (5) in plant samples and commercial products of A. formosanus. Interferences were removed by a simple Diaion HP-20 column chromatography. In the 1H NMR spectrum, the signals of compounds 1 (δ 2.88), 2 (δ 2.47), 3 (δ 1.12), 4 (δ 7.05) and 5 (δ 7.84) were selected and integrated as the target peaks, separated well from other signals in D2O. The developed 1H NMR method provides a rapid and simple quantitative method without any derivatization, pH control or addition of NMR shift reagent, and the analytical solvent system is very safe, convenient and simple.
| Original language | English |
|---|---|
| Pages (from-to) | 5645-5650 |
| Number of pages | 6 |
| Journal | Analytical Methods |
| Volume | 8 |
| Issue number | 28 |
| DOIs | |
| Publication status | Published - 2016 Jul 28 |
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All Science Journal Classification (ASJC) codes
- Analytical Chemistry
- Chemical Engineering(all)
- Engineering(all)
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A rapid quantitative 1H NMR analysis of kinsenoside and other bioactive principles from : Anoectochilus formosanus. / Hsieh, Li Ying; Chan, Hsiu Hui; Hung, Hsin-Yi; Kuo, Chao Lin; Wu, Jin Bin; Sun, I-Wen; Kuo, Ping-Chung; Wu, Tian-Shung.
In: Analytical Methods, Vol. 8, No. 28, 28.07.2016, p. 5645-5650.Research output: Contribution to journal › Article
TY - JOUR
T1 - A rapid quantitative 1H NMR analysis of kinsenoside and other bioactive principles from
T2 - Anoectochilus formosanus
AU - Hsieh, Li Ying
AU - Chan, Hsiu Hui
AU - Hung, Hsin-Yi
AU - Kuo, Chao Lin
AU - Wu, Jin Bin
AU - Sun, I-Wen
AU - Kuo, Ping-Chung
AU - Wu, Tian-Shung
PY - 2016/7/28
Y1 - 2016/7/28
N2 - Anoectochilus formosanus is a famous traditional Taiwanese folk medicine. Kinsenoside (1), one of the major active components in A. formosanus, exhibits hepatoprotective, antihyperliposis and antiflammatory activities. In this study, a proton nuclear magnetic resonance (1H NMR) method was developed for rapid quantitative analysis of several bioactive major constituents, including kinsenoside (1), 3-(R)-3-β-d-gluco-pyranosyloxy-4-hydroxybutanoic acid (2), 1-O-isopropyl-β-d-gluco-pyranoside (3), 4-(β-d-gluco-pyranosyloxy)benzyl alcohol (4) and benzoic acid (5) in plant samples and commercial products of A. formosanus. Interferences were removed by a simple Diaion HP-20 column chromatography. In the 1H NMR spectrum, the signals of compounds 1 (δ 2.88), 2 (δ 2.47), 3 (δ 1.12), 4 (δ 7.05) and 5 (δ 7.84) were selected and integrated as the target peaks, separated well from other signals in D2O. The developed 1H NMR method provides a rapid and simple quantitative method without any derivatization, pH control or addition of NMR shift reagent, and the analytical solvent system is very safe, convenient and simple.
AB - Anoectochilus formosanus is a famous traditional Taiwanese folk medicine. Kinsenoside (1), one of the major active components in A. formosanus, exhibits hepatoprotective, antihyperliposis and antiflammatory activities. In this study, a proton nuclear magnetic resonance (1H NMR) method was developed for rapid quantitative analysis of several bioactive major constituents, including kinsenoside (1), 3-(R)-3-β-d-gluco-pyranosyloxy-4-hydroxybutanoic acid (2), 1-O-isopropyl-β-d-gluco-pyranoside (3), 4-(β-d-gluco-pyranosyloxy)benzyl alcohol (4) and benzoic acid (5) in plant samples and commercial products of A. formosanus. Interferences were removed by a simple Diaion HP-20 column chromatography. In the 1H NMR spectrum, the signals of compounds 1 (δ 2.88), 2 (δ 2.47), 3 (δ 1.12), 4 (δ 7.05) and 5 (δ 7.84) were selected and integrated as the target peaks, separated well from other signals in D2O. The developed 1H NMR method provides a rapid and simple quantitative method without any derivatization, pH control or addition of NMR shift reagent, and the analytical solvent system is very safe, convenient and simple.
UR - http://www.scopus.com/inward/record.url?scp=84978764098&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84978764098&partnerID=8YFLogxK
U2 - 10.1039/c6ay00670a
DO - 10.1039/c6ay00670a
M3 - Article
AN - SCOPUS:84978764098
VL - 8
SP - 5645
EP - 5650
JO - Analytical Methods
JF - Analytical Methods
SN - 1759-9660
IS - 28
ER -