Anoectochilus formosanus is a famous traditional Taiwanese folk medicine. Kinsenoside (1), one of the major active components in A. formosanus, exhibits hepatoprotective, antihyperliposis and antiflammatory activities. In this study, a proton nuclear magnetic resonance (1H NMR) method was developed for rapid quantitative analysis of several bioactive major constituents, including kinsenoside (1), 3-(R)-3-β-d-gluco-pyranosyloxy-4-hydroxybutanoic acid (2), 1-O-isopropyl-β-d-gluco-pyranoside (3), 4-(β-d-gluco-pyranosyloxy)benzyl alcohol (4) and benzoic acid (5) in plant samples and commercial products of A. formosanus. Interferences were removed by a simple Diaion HP-20 column chromatography. In the 1H NMR spectrum, the signals of compounds 1 (δ 2.88), 2 (δ 2.47), 3 (δ 1.12), 4 (δ 7.05) and 5 (δ 7.84) were selected and integrated as the target peaks, separated well from other signals in D2O. The developed 1H NMR method provides a rapid and simple quantitative method without any derivatization, pH control or addition of NMR shift reagent, and the analytical solvent system is very safe, convenient and simple.
All Science Journal Classification (ASJC) codes
- Analytical Chemistry
- Chemical Engineering(all)