A rapid screening for hemoglobin-specific aptamers by using a continuous microfluidic system

Ching Chu Wu, Hsin I. Lin, Chen Hsun Weng, Shu Chu Shiesh, Gwo Bin Lee

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Citations (Scopus)

Abstract

Systematic evolution of ligands by exponential enrichment (SELEX) is a method to screen specific DNA- or RNA-based aptamers by repeated rounds of partition and amplification from random pool of oligonucleotides. The screening products have high binding affinity for the target molecule, which can be an excellent biomarker for diagnosis and therapeutics. This study reports a new continuous microfluidic system to perform SELEX in an automatic mode, which is an excellent tool for fast selection of aptamers. As a demonstration, hemoglomin-specific aptamer was screened. The screened aptamer can be used for fast diagnosis of hemoglobin, which is a crucial indicator for diabetes mellitus.

Original languageEnglish
Title of host publicationProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
PublisherChemical and Biological Microsystems Society
Pages353-355
Number of pages3
ISBN (Print)9780979806452
Publication statusPublished - 2012 Jan 1
Event16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 - Okinawa, Japan
Duration: 2012 Oct 282012 Nov 1

Publication series

NameProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012

Other

Other16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
CountryJapan
CityOkinawa
Period12-10-2812-11-01

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All Science Journal Classification (ASJC) codes

  • Chemical Engineering (miscellaneous)
  • Bioengineering

Cite this

Wu, C. C., Lin, H. I., Weng, C. H., Shiesh, S. C., & Lee, G. B. (2012). A rapid screening for hemoglobin-specific aptamers by using a continuous microfluidic system. In Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 (pp. 353-355). (Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012). Chemical and Biological Microsystems Society.