A reference map and identification of porcine testis proteins using 2-DE and MS

San Yuan Huang, Jyh Hung Lin, Yu Huey Chen, Chin Kai Chuang, En Chung Lin, Mu Chiou Huang, Hsiao Fang Sunny Sun, Wen Chuan Lee

Research output: Contribution to journalArticlepeer-review

46 Citations (Scopus)

Abstract

The development of the testis is essential for maturation of male mammals. A complete understanding of proteins expressed in the testis will provide biological information on many reproductive dysfunctions in males. The purposes of this study were to apply a proteomic approach to investigating protein composition and to establish a 2-D PAGE reference map for porcine testis proteins. MALDI-TOF MS was performed for protein identification. When 1 mg of total proteins was assayed by 2-D PAGE and stained with colloidal CBB, more than 400 proteins with a pI of pH 3-10 and Mr of 10-200 kDa could be detected. Protein expression varied among individuals, with CV between 4.7 and 131.5%. A total of 447 protein spots were excised for identification, among which 337 spots were identified by searching the mass spectra against the NCBInr database. Identification of the remaining 110 spots was unsuccessful. A 2-D PAGE-based porcine testis protein database has been constructed on the basis of the results and will be published on the WWW. This database should be valuable for investigating the developmental biology and pathology of porcine testis.

Original languageEnglish
Pages (from-to)4205-4212
Number of pages8
JournalProteomics
Volume5
Issue number16
DOIs
Publication statusPublished - 2005 Nov 1

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

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