A role of ygfZ in the Escherichia coli response to plumbagin challenge

Ching Nan Lin, Wan Jr Syu, Wei Sheng W. Sun, Jenn Wei Chen, Tai Hung Chen, Ming Jaw Don, Shao Hung Wang

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Plumbagin is found in many herbal plants and inhibits the growth of various bacteria. Escherichia coli strains are relatively resistant to this drug. The mechanism of resistance is not clear. Previous findings showed that plumbagin treatment triggered up-regulation of many genes in E. coli including ahpC, mdaB, nfnB, nfo, sodA, yggX and ygfZ. By analyzing minimal inhibition concentration and inhibition zones of plumbagin in various gene-disruption mutants, ygfZ and sodA were found critical for the bacteria to resist plumbagin toxicity. We also found that the roles of YgfZ and SodA in detoxifying plumbagin are independent of each other. This is because of the fact that ectopically expressed SodA reduced the superoxide stress but not restore the resistance of bacteria when encountering plumbagin at the absence of ygfZ. On the other hand, an ectopically expressed YgfZ was unable to complement and failed to rescue the plumbagin resistance when sodA was perturbed. Furthermore, mutagenesis analysis showed that residue Cys228 within YgfZ fingerprint region was critical for the resistance of E. coli to plumbagin. By solvent extraction and HPLC analysis to follow the fate of the chemical, it was found that plumbagin vanished apparently from the culture of YgfZ-expressing E. coli. A less toxic form, methylated plumbagin, which may represent one of the YgfZ-dependent metabolites, was found in the culture supernatant of the wild type E. coli but not in the ygfZ mutant. Our results showed that the presence of ygfZ is not only critical for the E coli resistance to plumbagin but also facilitates the plumbagin degradation.

Original languageEnglish
Article number84
JournalJournal of biomedical science
Volume17
Issue number1
DOIs
Publication statusPublished - 2010 Nov 11

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology
  • Biochemistry, medical
  • Pharmacology (medical)

Fingerprint Dive into the research topics of 'A role of ygfZ in the Escherichia coli response to plumbagin challenge'. Together they form a unique fingerprint.

  • Cite this

    Lin, C. N., Syu, W. J., Sun, W. S. W., Chen, J. W., Chen, T. H., Don, M. J., & Wang, S. H. (2010). A role of ygfZ in the Escherichia coli response to plumbagin challenge. Journal of biomedical science, 17(1), [84]. https://doi.org/10.1186/1423-0127-17-84