A sensitive and selective magnetic graphene composite-modified polycrystalline-silicon nanowire field-effect transistor for bladder cancer diagnosis

Hsiao Chien Chen, Yi Ting Chen, Rung Ywan Tsai, Min Cheng Chen, Shi Liang Chen, Min Cong Xiao, Chien Lun Chen, Mu Yi Hua

Research output: Contribution to journalArticlepeer-review

30 Citations (Scopus)

Abstract

In this study, we describe the urinary quantification of apolipoprotein A II protein (APOA2 protein), a biomarker for the diagnosis of bladder cancer, using an n-type polycrystalline silicon nanowire field-effect transistor (poly-SiNW-FET). The modification of poly-SiNW-FET by magnetic graphene with long-chain acid groups (MGLA) synthesized via Friedel-Crafts acylation was compared with that obtained using short-chain acid groups (MGSA). Compared with MGSA, the MGLA showed a higher immobilization degree and bioactivity to the anti-APOA2 antibody (Ab) due to its lower steric hindrance. In addition, the magnetic properties enabled rapid separation and purification during Ab immobilization, ultimately preserving its bioactivity. The Ab-MGLA/poly-SiNW-FET exhibited a linear dependence of relative response to the logarithmical concentration in a range between 19.5pgmL-1 and 1.95μgmL-1, with a limit of detection (LOD) of 6.7pgmL-1. An additional washing step before measurement aimed at excluding the interfering biocomponents ensured the reliability of the assay. We conclude that our biosensor efficiently distinguishes mean values of urinary APOA2 protein concentrations between patients with bladder cancer (29-344ngmL-1) and those with hernia (0.425-9.47ngmL-1).

Original languageEnglish
Pages (from-to)198-207
Number of pages10
JournalBiosensors and Bioelectronics
Volume66
DOIs
Publication statusPublished - 2015 Apr 5

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

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