A simple and quantitative method for analysis of methoxyamine by capillary zone electrophoresis

Yu Chieh Liao, Mei Jywan Syu, Yan Xu

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Methoxyamine (MX) is a potential new chemotherapeutic agent. In structure, MX lacks a chromophore for spectrophotometric detection. Derivatization of MX with chromophoric 4-(diethylamino)benzaldehyde (DEAB) was performed and the resultant MX derivative (DBMOH+) can be analyzed by capillary zone electrophoresis (CZE) with ultraviolet (UV) detection at 200 nm. The limit of detection (SD/S = 3.3) of the method for MX was 2.50 μM with a 2 s injection at the pressure of 3.45 kPa. The linear calibration range for MX was 5.00-500 μM using N,N-dimethyl-p-toluidine (DMPT) as the internal standard. The intra- and inter-assay precision and accuracy of the method were ≤4% and within 98.4-104%, respectively. This method may be used for the quantitative determination of MX in pharmaceutical preparations.

Original languageEnglish
Pages (from-to)2433-2443
Number of pages11
JournalJournal of Liquid Chromatography and Related Technologies
Issue number15
Publication statusPublished - 2005 Oct 4


All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Pharmaceutical Science
  • Clinical Biochemistry

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