A simple electrokinetic protein preconcentrator utilizing nano-interstices

Yu Hung Chen; Hsuan Franziska Wu; Tamara G. Amstislavskaya; Chang Yu Li; Chun Ping Jen

doi:10.1063/1.4946768

A simple electrokinetic protein preconcentrator utilizing nano-interstices

Yu Hung Chen, Hsuan Franziska Wu, Tamara G. Amstislavskaya, Chang Yu Li, Chun Ping Jen

School of Medicine

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

This work proposes a simple method for creating nanofluidic channels for protein preconcentration through self-assembled gold nanoparticles (AuNPs) using the exclusion-enrichment effect. A depletion force is elicited in nano-interstices among self-assembled AuNPs due to the overlap of electrical double layers (EDLs); therefore, proteins quickly accumulate. The experimental results show that the generation of depletion forces is correlated with the size of the AuNPs. The self-assembled monolayer of AuNPs (13 nm in diameter) can successfully preconcentrate proteins through effective EDL overlapping. This approach provides a new process to produce nanochannels that does not require high-voltage or time-consuming fabrication.

Original language	English
Article number	024121
Journal	Biomicrofluidics
Volume	10
Issue number	2
DOIs	https://doi.org/10.1063/1.4946768
Publication status	Published - 2016 Mar 1

All Science Journal Classification (ASJC) codes

Biomedical Engineering
Materials Science(all)
Condensed Matter Physics
Fluid Flow and Transfer Processes
Colloid and Surface Chemistry

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10.1063/1.4946768

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abstract = "This work proposes a simple method for creating nanofluidic channels for protein preconcentration through self-assembled gold nanoparticles (AuNPs) using the exclusion-enrichment effect. A depletion force is elicited in nano-interstices among self-assembled AuNPs due to the overlap of electrical double layers (EDLs); therefore, proteins quickly accumulate. The experimental results show that the generation of depletion forces is correlated with the size of the AuNPs. The self-assembled monolayer of AuNPs (13 nm in diameter) can successfully preconcentrate proteins through effective EDL overlapping. This approach provides a new process to produce nanochannels that does not require high-voltage or time-consuming fabrication.",

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AU - Chen, Yu Hung

AU - Wu, Hsuan Franziska

AU - Amstislavskaya, Tamara G.

AU - Li, Chang Yu

AU - Jen, Chun Ping

N1 - Funding Information: The authors would like to thank the National Science Council of the Republic of China, Taiwan, for its financial support of this research under the following Grant Nos.: MOST 104-2221-E-194-007-and MOST 104-2633-B-006-002-. Publisher Copyright: © 2016 Author(s).

PY - 2016/3/1

Y1 - 2016/3/1

N2 - This work proposes a simple method for creating nanofluidic channels for protein preconcentration through self-assembled gold nanoparticles (AuNPs) using the exclusion-enrichment effect. A depletion force is elicited in nano-interstices among self-assembled AuNPs due to the overlap of electrical double layers (EDLs); therefore, proteins quickly accumulate. The experimental results show that the generation of depletion forces is correlated with the size of the AuNPs. The self-assembled monolayer of AuNPs (13 nm in diameter) can successfully preconcentrate proteins through effective EDL overlapping. This approach provides a new process to produce nanochannels that does not require high-voltage or time-consuming fabrication.

AB - This work proposes a simple method for creating nanofluidic channels for protein preconcentration through self-assembled gold nanoparticles (AuNPs) using the exclusion-enrichment effect. A depletion force is elicited in nano-interstices among self-assembled AuNPs due to the overlap of electrical double layers (EDLs); therefore, proteins quickly accumulate. The experimental results show that the generation of depletion forces is correlated with the size of the AuNPs. The self-assembled monolayer of AuNPs (13 nm in diameter) can successfully preconcentrate proteins through effective EDL overlapping. This approach provides a new process to produce nanochannels that does not require high-voltage or time-consuming fabrication.

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A simple electrokinetic protein preconcentrator utilizing nano-interstices

Abstract

All Science Journal Classification (ASJC) codes

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