TY - GEN
T1 - A Study on Enhanced Fluorescence Signal-to-noise by using the Stray Light Shutter for Quantitative PCR Chip
AU - Chao, Liang Chieh
AU - Chou, Chun Han
AU - Tsai, Hsin Yi
AU - Huang, Kuo Cheng
AU - Shieh, Dar Bin
N1 - Publisher Copyright:
© 2021 by SCITEPRESS – Science and Technology Publications, Lda. All rights reserved.
PY - 2021
Y1 - 2021
N2 - Quantitative polymerase chain reaction (qPCR) is the most important inspection technique for virus, especially for coronavirus disease, in this year. The qPCR chip and device were planned to develop because of the characteristics of fast inspection time, high accuracy and small system volume. Therein, the fluorescence intensity was the important signal in qPCR device, which represented the positive or negative reaction after the DNA was amplified and bound on the fluorescence dye, but the fluorescence signal was easily to be affected by the excitation and scattered light. The mini spectrometer was employed to receive the fluorescence intensity in PCR chip in this study, and the optical simulation was progressed, and the stray light shutter (SLS) was added to improve the signal-to-noise ratio (SNR) of fluorescence. The analysis results showed that the SNR of fluorescence can be enhanced from 3.14 to 16.78 by using the SLS with shape of extend component aperture, which the protruding structure was at the direction away from center. The results from this manuscript can provide the important reference information to the developer of qPCR chip, whom can obtain the high SNR fluorescence signal in qPCR inspection process for disease.
AB - Quantitative polymerase chain reaction (qPCR) is the most important inspection technique for virus, especially for coronavirus disease, in this year. The qPCR chip and device were planned to develop because of the characteristics of fast inspection time, high accuracy and small system volume. Therein, the fluorescence intensity was the important signal in qPCR device, which represented the positive or negative reaction after the DNA was amplified and bound on the fluorescence dye, but the fluorescence signal was easily to be affected by the excitation and scattered light. The mini spectrometer was employed to receive the fluorescence intensity in PCR chip in this study, and the optical simulation was progressed, and the stray light shutter (SLS) was added to improve the signal-to-noise ratio (SNR) of fluorescence. The analysis results showed that the SNR of fluorescence can be enhanced from 3.14 to 16.78 by using the SLS with shape of extend component aperture, which the protruding structure was at the direction away from center. The results from this manuscript can provide the important reference information to the developer of qPCR chip, whom can obtain the high SNR fluorescence signal in qPCR inspection process for disease.
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U2 - 10.5220/0010362500540060
DO - 10.5220/0010362500540060
M3 - Conference contribution
AN - SCOPUS:85173929596
SN - 9789897584923
T3 - International Conference on Photonics, Optics and Laser Technology
SP - 54
EP - 60
BT - PHOTOPTICS 2021 - Proceedings of the 9th International Conference on Photonics, Optics and Laser Technology
A2 - Raposo, Maria
A2 - Ribeiro, Paulo A.
PB - Science and Technology Publications, Lda
T2 - 9th International Conference on Photonics, Optics and Laser Technology, PHOTOPTICS 2021
Y2 - 11 February 2021 through 13 February 2021
ER -