TY - JOUR
T1 - A suction-type microfluidic immunosensing chip for rapid detection of the dengue virus
AU - Weng, Chen Hsun
AU - Huang, Tze Bin
AU - Huang, Chih Chia
AU - Yeh, Chen Sheng
AU - Lei, Huan Yao
AU - Lee, Gwo Bin
N1 - Funding Information:
Acknowledgements The authors gratefully acknowledge the financial support provided to this study by the National Science Council in Taiwan (NSC 98-2627-B-006-006; NSC 98-2120-M-006-001).
PY - 2011/6
Y1 - 2011/6
N2 - The enzyme-linked immunosorbent assay (ELISA) is widely used in medical diagnostics. In order to reduce the diagnosis time and to lower the consumption of sample/reagents in an ELISA assay, a suction-type, automatic, pneumatically-driven microfluidic chip has been designed and fabricated in this study. The microfluidic chip integrates a multi-functional micro-transport/ mixing unit, for transporting metering and mixing of samples and reagents in order to automatically perform the entire ELISA protocol. A new surface modification has been adopted which allows for a high processing capacity. The detection sensitivity for the dengue virus is found to be 101 PFU/ml, which is much better than a conventional ELISA assay (103 PFU/ml). The entire assay time is only 30 min, which is much faster than with 96-well microtiter plates (4 h). The consumed sample and reagent volume is only 12 μl, which is less than a conventional assay (100 μl). The development of this microfluidic chip may be promising for other immunosensing applications.
AB - The enzyme-linked immunosorbent assay (ELISA) is widely used in medical diagnostics. In order to reduce the diagnosis time and to lower the consumption of sample/reagents in an ELISA assay, a suction-type, automatic, pneumatically-driven microfluidic chip has been designed and fabricated in this study. The microfluidic chip integrates a multi-functional micro-transport/ mixing unit, for transporting metering and mixing of samples and reagents in order to automatically perform the entire ELISA protocol. A new surface modification has been adopted which allows for a high processing capacity. The detection sensitivity for the dengue virus is found to be 101 PFU/ml, which is much better than a conventional ELISA assay (103 PFU/ml). The entire assay time is only 30 min, which is much faster than with 96-well microtiter plates (4 h). The consumed sample and reagent volume is only 12 μl, which is less than a conventional assay (100 μl). The development of this microfluidic chip may be promising for other immunosensing applications.
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U2 - 10.1007/s10544-011-9529-3
DO - 10.1007/s10544-011-9529-3
M3 - Article
C2 - 21448655
AN - SCOPUS:79958173601
SN - 1387-2176
VL - 13
SP - 585
EP - 595
JO - Biomedical Microdevices
JF - Biomedical Microdevices
IS - 3
ER -
