A suction-type microfluidic immunosensing chip for rapid detection of the dengue virus

Chen Hsun Weng, Tze Bin Huang, Chih Chia Huang, Chen Sheng Yeh, Huan Yao Lei, Gwo Bin Lee

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)

Abstract

The enzyme-linked immunosorbent assay (ELISA) is widely used in medical diagnostics. In order to reduce the diagnosis time and to lower the consumption of sample/reagents in an ELISA assay, a suction-type, automatic, pneumatically-driven microfluidic chip has been designed and fabricated in this study. The microfluidic chip integrates a multi-functional micro-transport/ mixing unit, for transporting metering and mixing of samples and reagents in order to automatically perform the entire ELISA protocol. A new surface modification has been adopted which allows for a high processing capacity. The detection sensitivity for the dengue virus is found to be 101 PFU/ml, which is much better than a conventional ELISA assay (103 PFU/ml). The entire assay time is only 30 min, which is much faster than with 96-well microtiter plates (4 h). The consumed sample and reagent volume is only 12 μl, which is less than a conventional assay (100 μl). The development of this microfluidic chip may be promising for other immunosensing applications.

Original languageEnglish
Pages (from-to)585-595
Number of pages11
JournalBiomedical Microdevices
Volume13
Issue number3
DOIs
Publication statusPublished - 2011 Jun 1

All Science Journal Classification (ASJC) codes

  • Biomedical Engineering
  • Molecular Biology

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