A suction-type, pneumatic microfluidic device for rapid DNA extraction

Sung Yi Yang, Chen-Hsun Weng, Gwo Bin Lee

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This current study presents a new miniature, integrated system capable of rapid extraction of genomic DNA (gDNA) from saliva samples. Three major components of the DNA extraction chip, including six symmetrical, normally-closed microvalves, a sample transport/mixing unit and a waste unit are integrated in this device. The microfluidic DNA extraction chip was made of CNC machining and PDMS casting processes to integrate a liquid channel layer, an air chamber layer and a glass layer on a single chip. Liquid samples can be transported and mixed by the suction-type sample transport/mixing unit by using micropumps, micromixers and normally-closed microvalves. Experimental results showed the pumping rate can be as high as 400 μL/min at an applied pressure of -70 KPa for an air chamber with a depth of 1.5 mm. A mixing efficiency as high as 96.5% can be achieved within 3 sec. Experimental data also showed that gDNA with an average concentration of 45 ± 3 ng/L and an optical intensity (OD) value (260/280) of 1.5 ± 0.2 from ten measurements can be successfully achieved within 25 minutes. Consequently, the proposed miniature system can provide a powerful platform for automatic, rapid DNA extraction.

Original languageEnglish
Title of host publicationNEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems
Pages71-74
Number of pages4
DOIs
Publication statusPublished - 2011 Oct 4
Event6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2011 - Kaohsiung, Taiwan
Duration: 2011 Feb 202011 Feb 23

Publication series

NameNEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems

Other

Other6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2011
CountryTaiwan
CityKaohsiung
Period11-02-2011-02-23

Fingerprint

microfluidic devices
pneumatics
suction
deoxyribonucleic acid
chips
chambers
saliva
air
liquids
machining
pumping
platforms
glass

All Science Journal Classification (ASJC) codes

  • Atomic and Molecular Physics, and Optics

Cite this

Yang, S. Y., Weng, C-H., & Lee, G. B. (2011). A suction-type, pneumatic microfluidic device for rapid DNA extraction. In NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems (pp. 71-74). [6017298] (NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems). https://doi.org/10.1109/NEMS.2011.6017298
Yang, Sung Yi ; Weng, Chen-Hsun ; Lee, Gwo Bin. / A suction-type, pneumatic microfluidic device for rapid DNA extraction. NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems. 2011. pp. 71-74 (NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems).
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abstract = "This current study presents a new miniature, integrated system capable of rapid extraction of genomic DNA (gDNA) from saliva samples. Three major components of the DNA extraction chip, including six symmetrical, normally-closed microvalves, a sample transport/mixing unit and a waste unit are integrated in this device. The microfluidic DNA extraction chip was made of CNC machining and PDMS casting processes to integrate a liquid channel layer, an air chamber layer and a glass layer on a single chip. Liquid samples can be transported and mixed by the suction-type sample transport/mixing unit by using micropumps, micromixers and normally-closed microvalves. Experimental results showed the pumping rate can be as high as 400 μL/min at an applied pressure of -70 KPa for an air chamber with a depth of 1.5 mm. A mixing efficiency as high as 96.5{\%} can be achieved within 3 sec. Experimental data also showed that gDNA with an average concentration of 45 ± 3 ng/L and an optical intensity (OD) value (260/280) of 1.5 ± 0.2 from ten measurements can be successfully achieved within 25 minutes. Consequently, the proposed miniature system can provide a powerful platform for automatic, rapid DNA extraction.",
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Yang, SY, Weng, C-H & Lee, GB 2011, A suction-type, pneumatic microfluidic device for rapid DNA extraction. in NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems., 6017298, NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, pp. 71-74, 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2011, Kaohsiung, Taiwan, 11-02-20. https://doi.org/10.1109/NEMS.2011.6017298

A suction-type, pneumatic microfluidic device for rapid DNA extraction. / Yang, Sung Yi; Weng, Chen-Hsun; Lee, Gwo Bin.

NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems. 2011. p. 71-74 6017298 (NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Yang SY, Weng C-H, Lee GB. A suction-type, pneumatic microfluidic device for rapid DNA extraction. In NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems. 2011. p. 71-74. 6017298. (NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems). https://doi.org/10.1109/NEMS.2011.6017298