TY - GEN
T1 - A suction-type, pneumatic microfluidic device for rapid DNA extraction
AU - Yang, Sung Yi
AU - Weng, Chen Hsun
AU - Lee, Gwo Bin
PY - 2011/10/4
Y1 - 2011/10/4
N2 - This current study presents a new miniature, integrated system capable of rapid extraction of genomic DNA (gDNA) from saliva samples. Three major components of the DNA extraction chip, including six symmetrical, normally-closed microvalves, a sample transport/mixing unit and a waste unit are integrated in this device. The microfluidic DNA extraction chip was made of CNC machining and PDMS casting processes to integrate a liquid channel layer, an air chamber layer and a glass layer on a single chip. Liquid samples can be transported and mixed by the suction-type sample transport/mixing unit by using micropumps, micromixers and normally-closed microvalves. Experimental results showed the pumping rate can be as high as 400 μL/min at an applied pressure of -70 KPa for an air chamber with a depth of 1.5 mm. A mixing efficiency as high as 96.5% can be achieved within 3 sec. Experimental data also showed that gDNA with an average concentration of 45 ± 3 ng/L and an optical intensity (OD) value (260/280) of 1.5 ± 0.2 from ten measurements can be successfully achieved within 25 minutes. Consequently, the proposed miniature system can provide a powerful platform for automatic, rapid DNA extraction.
AB - This current study presents a new miniature, integrated system capable of rapid extraction of genomic DNA (gDNA) from saliva samples. Three major components of the DNA extraction chip, including six symmetrical, normally-closed microvalves, a sample transport/mixing unit and a waste unit are integrated in this device. The microfluidic DNA extraction chip was made of CNC machining and PDMS casting processes to integrate a liquid channel layer, an air chamber layer and a glass layer on a single chip. Liquid samples can be transported and mixed by the suction-type sample transport/mixing unit by using micropumps, micromixers and normally-closed microvalves. Experimental results showed the pumping rate can be as high as 400 μL/min at an applied pressure of -70 KPa for an air chamber with a depth of 1.5 mm. A mixing efficiency as high as 96.5% can be achieved within 3 sec. Experimental data also showed that gDNA with an average concentration of 45 ± 3 ng/L and an optical intensity (OD) value (260/280) of 1.5 ± 0.2 from ten measurements can be successfully achieved within 25 minutes. Consequently, the proposed miniature system can provide a powerful platform for automatic, rapid DNA extraction.
UR - http://www.scopus.com/inward/record.url?scp=80053339529&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=80053339529&partnerID=8YFLogxK
U2 - 10.1109/NEMS.2011.6017298
DO - 10.1109/NEMS.2011.6017298
M3 - Conference contribution
AN - SCOPUS:80053339529
SN - 9781612847757
T3 - NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems
SP - 71
EP - 74
BT - NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems
T2 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2011
Y2 - 20 February 2011 through 23 February 2011
ER -