A two-stage bioprocess for hydrogen and methane production from rice straw bioethanol residues

Hai Hsuan Cheng, Liang Ming Whang, Chao Wei Wu, Man Chien Chung

Research output: Contribution to journalArticlepeer-review

39 Citations (Scopus)


This study evaluates a two-stage bioprocess for recovering hydrogen and methane while treating organic residues of fermentative bioethanol from rice straw. The obtained results indicate that controlling a proper volumetric loading rate, substrate-to-biomass ratio, or F/M ratio is important to maximizing biohydrogen production from rice straw bioethanol residues. Clostridium tyrobutyricum, the identified major hydrogen-producing bacteria enriched in the hydrogen bioreactor, is likely utilizing lactate and acetate for biohydrogen production. The occurrence of acetogenesis during biohydrogen fermentation may reduce the B/A ratio and lead to a lower hydrogen production. Organic residues remained in the effluent of hydrogen bioreactor can be effectively converted to methane with a rate of 2.8mmol CH 4/gVSS/h at VLR of 4.6kgCOD/m 3/d. Finally, approximately 75% of COD in rice straw bioethanol residues can be removed and among that 1.3% and 66.1% of COD can be recovered in the forms of hydrogen and methane, respectively.

Original languageEnglish
Pages (from-to)23-29
Number of pages7
JournalBioresource technology
Publication statusPublished - 2012 Jun

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal


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