A validated HPLC method with ultraviolet detection for the determination of buformin in plasma

Chen-Hsi Chou, Ching Ling Cheng, Chien Chen Huang

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A sensitive and rapid high-performance liquid chromatographic assay is developed and validated for the determination of buformin in plasma. After addition of metformin as the internal standard, the analytes were deproteinated with acetonitrile, washed with dichloromethane, and the resulting supernatant injected. Chromatography was performed at ambient temperature by pumping a mobile phase of 0.03 M diammonium hydrogen phosphate buffer (pH 7, 250 mL) in methanol (750 mL) at a flow rate of 1 mL/min through a silica column. Buformin and metformin were detected at 236 nm, and eluted 9.8 and 15.4 min, respectively. No endogenous substances were found to interfere. Calibration curves were linear over the concentration range of 20-2000 ng/mL. The limit of quantitation was 20 ng/mL. The intra- and inter-day relative standard deviation (RSD) was 8.3%, or less, and the accuracy was within 10.1% of the relative error (RE). The method is suitable in pharmacokinetic investigation of buformin.

Original languageEnglish
Pages (from-to)254-258
Number of pages5
JournalBiomedical Chromatography
Volume18
Issue number4
DOIs
Publication statusPublished - 2004 May 1

Fingerprint

Buformin
Metformin
High Pressure Liquid Chromatography
Plasmas
Pharmacokinetics
Methylene Chloride
Chromatography
Silicon Dioxide
Cell Division
Calibration
Methanol
Assays
Buffers
Flow rate
Temperature
Liquids

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Molecular Biology
  • Pharmacology
  • Drug Discovery
  • Clinical Biochemistry

Cite this

@article{350c685d140f480789446c458dfc2d73,
title = "A validated HPLC method with ultraviolet detection for the determination of buformin in plasma",
abstract = "A sensitive and rapid high-performance liquid chromatographic assay is developed and validated for the determination of buformin in plasma. After addition of metformin as the internal standard, the analytes were deproteinated with acetonitrile, washed with dichloromethane, and the resulting supernatant injected. Chromatography was performed at ambient temperature by pumping a mobile phase of 0.03 M diammonium hydrogen phosphate buffer (pH 7, 250 mL) in methanol (750 mL) at a flow rate of 1 mL/min through a silica column. Buformin and metformin were detected at 236 nm, and eluted 9.8 and 15.4 min, respectively. No endogenous substances were found to interfere. Calibration curves were linear over the concentration range of 20-2000 ng/mL. The limit of quantitation was 20 ng/mL. The intra- and inter-day relative standard deviation (RSD) was 8.3{\%}, or less, and the accuracy was within 10.1{\%} of the relative error (RE). The method is suitable in pharmacokinetic investigation of buformin.",
author = "Chen-Hsi Chou and Cheng, {Ching Ling} and Huang, {Chien Chen}",
year = "2004",
month = "5",
day = "1",
doi = "10.1002/bmc.312",
language = "English",
volume = "18",
pages = "254--258",
journal = "Biomedical Chromatography",
issn = "0269-3879",
publisher = "John Wiley and Sons Ltd",
number = "4",

}

A validated HPLC method with ultraviolet detection for the determination of buformin in plasma. / Chou, Chen-Hsi; Cheng, Ching Ling; Huang, Chien Chen.

In: Biomedical Chromatography, Vol. 18, No. 4, 01.05.2004, p. 254-258.

Research output: Contribution to journalArticle

TY - JOUR

T1 - A validated HPLC method with ultraviolet detection for the determination of buformin in plasma

AU - Chou, Chen-Hsi

AU - Cheng, Ching Ling

AU - Huang, Chien Chen

PY - 2004/5/1

Y1 - 2004/5/1

N2 - A sensitive and rapid high-performance liquid chromatographic assay is developed and validated for the determination of buformin in plasma. After addition of metformin as the internal standard, the analytes were deproteinated with acetonitrile, washed with dichloromethane, and the resulting supernatant injected. Chromatography was performed at ambient temperature by pumping a mobile phase of 0.03 M diammonium hydrogen phosphate buffer (pH 7, 250 mL) in methanol (750 mL) at a flow rate of 1 mL/min through a silica column. Buformin and metformin were detected at 236 nm, and eluted 9.8 and 15.4 min, respectively. No endogenous substances were found to interfere. Calibration curves were linear over the concentration range of 20-2000 ng/mL. The limit of quantitation was 20 ng/mL. The intra- and inter-day relative standard deviation (RSD) was 8.3%, or less, and the accuracy was within 10.1% of the relative error (RE). The method is suitable in pharmacokinetic investigation of buformin.

AB - A sensitive and rapid high-performance liquid chromatographic assay is developed and validated for the determination of buformin in plasma. After addition of metformin as the internal standard, the analytes were deproteinated with acetonitrile, washed with dichloromethane, and the resulting supernatant injected. Chromatography was performed at ambient temperature by pumping a mobile phase of 0.03 M diammonium hydrogen phosphate buffer (pH 7, 250 mL) in methanol (750 mL) at a flow rate of 1 mL/min through a silica column. Buformin and metformin were detected at 236 nm, and eluted 9.8 and 15.4 min, respectively. No endogenous substances were found to interfere. Calibration curves were linear over the concentration range of 20-2000 ng/mL. The limit of quantitation was 20 ng/mL. The intra- and inter-day relative standard deviation (RSD) was 8.3%, or less, and the accuracy was within 10.1% of the relative error (RE). The method is suitable in pharmacokinetic investigation of buformin.

UR - http://www.scopus.com/inward/record.url?scp=3042526488&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=3042526488&partnerID=8YFLogxK

U2 - 10.1002/bmc.312

DO - 10.1002/bmc.312

M3 - Article

VL - 18

SP - 254

EP - 258

JO - Biomedical Chromatography

JF - Biomedical Chromatography

SN - 0269-3879

IS - 4

ER -