TY - JOUR
T1 - Aberrant keratinization of reticular oral lichen planus is related to elastolysis
AU - Liao, Shih Chung
AU - Hsieh, Po Chen
AU - Huang, Jehn Shyun
AU - Hsu, Chia Wen
AU - Yuan, Kuo
N1 - Funding Information:
This study was partly supported by grant NSC 99-2314-B-006-035-MY3 from the National Science Council and grant NCKUH-10004002 from the National Cheng Kung University Hospital , Taiwan. The authors declare that there are no conflicts of interest in this study.
PY - 2012/6
Y1 - 2012/6
N2 - Objective. Aberrant keratinization is common in oral lichen planus (OLP) on buccal mucosa. Because elastin is crucial for maintaining the nonkeratinized phenotype of oral mucosa, we examined whether inflammatory elastases and the accompanying elastolysis were related to this feature. Study Design. Protein and mRNA levels of keratinization-associated keratins (K1/10), nonkeratinization- associated keratins (K4/13), elastin, neutrophil elastase, and macrophage metalloelastase (MMP-12) were compared between normal alveolar mucosa and reticular OLP from buccal mucosa. Normal alveolar mucosae were cultured ex vivo on an organ culture system with and without elastase treatment. After 14 days, the mucosae were examined for 4 keratin expressions. Results. The expressions of K1/10 and elastases increased, whereas those of K4/13 and elastin decreased in OLP. The nonkeratinized mucosa in the organ culture began to express K1/10 when elastase degraded the inherent elastin. Conclusions. Our study demonstrated that elastolysis in reticular OLP may be related to its aberrant keratinization.
AB - Objective. Aberrant keratinization is common in oral lichen planus (OLP) on buccal mucosa. Because elastin is crucial for maintaining the nonkeratinized phenotype of oral mucosa, we examined whether inflammatory elastases and the accompanying elastolysis were related to this feature. Study Design. Protein and mRNA levels of keratinization-associated keratins (K1/10), nonkeratinization- associated keratins (K4/13), elastin, neutrophil elastase, and macrophage metalloelastase (MMP-12) were compared between normal alveolar mucosa and reticular OLP from buccal mucosa. Normal alveolar mucosae were cultured ex vivo on an organ culture system with and without elastase treatment. After 14 days, the mucosae were examined for 4 keratin expressions. Results. The expressions of K1/10 and elastases increased, whereas those of K4/13 and elastin decreased in OLP. The nonkeratinized mucosa in the organ culture began to express K1/10 when elastase degraded the inherent elastin. Conclusions. Our study demonstrated that elastolysis in reticular OLP may be related to its aberrant keratinization.
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U2 - 10.1016/j.oooo.2012.02.007
DO - 10.1016/j.oooo.2012.02.007
M3 - Article
C2 - 22668709
AN - SCOPUS:84862561537
SN - 2212-4403
VL - 113
SP - 808
EP - 816
JO - Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology
JF - Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology
IS - 6
ER -