Aberrant RNA splicing in RHD 7-9 exons of DEL individuals in Taiwan: A mechanism study

Hsiang Chun Liu, Hock Liew Eng, Yu Fen Yang, Ya Hui Wang, Kuan Tsou Lin, Hua-Lin Wu, Tsun Mei Lin

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Background: The Rh blood D group provides a clinically important model of aberrant splicing with skipped exons. Approximately 30% of serologically D-negative Chinese individuals have an intact RHD gene (DEL phenotype) and induce allo-immunization in transfusions. The RHD1227GNA polymorphism occurs in >95% DEL phenotype of Asian descent. The effects of RHD 1227A and a novel allele on exon 9 splicing were examined. Results: Amplified DEL RNA products revealed that 3 transcripts inved skipping of exons 8-9, exon 9, or exon 9 with an inserted 170-bp cryptic exon located between exons 7 and 8. A novel, single nucleotide polymorphism was identified in the 7th intron, (IVS7) 923C>T, and present in all DEL patients. The odds ratio of RHD1227G>A allele with DEL phenotype was 2711. Splicing analysis of transcripts from minigenes containing the 1227GNA allele, but not the (IVS7) 923C>T allele, demonstrated aberrant exon 9 skipping. Conclusions: A combined haplotype of 1227G>A and IVS7 923C>T alleles was apparent in >95% DEL Chinese individuals. RHD1227A mutation significantly increased aberrant mRNA splicing, producing a hybrid RHD mRNA lacking exon 9. These results provide a molecular basis of the DEL phenotype in the Chinese population.

Original languageEnglish
Pages (from-to)565-573
Number of pages9
JournalBiochimica et Biophysica Acta - General Subjects
Volume1800
Issue number6
DOIs
Publication statusPublished - 2010 Jun 1

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology

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