Aberrantly expressed AURKC enhances the transformation and tumourigenicity of epithelial cells

Jen Hui Tsou, Kung-Chao Chang, Pey Yi Chang-Liao, Shu Ting Yang, Chung-Ta Lee, Ya-Ping Chen, Yi Chao Lee, Po-Wen Lin, Jenq-Chang Lee, Meng-Ru Shen, Chin Kai Chuang, Wen Chang Chang, Ju-Ming Wang, Liang-Yi Hung

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Over-expression of AURKC has been detected in human colorectal cancers, thyroid carcinoma and several cancer cell lines. However, the regulation and clinical implications of over-expressed AURKC in cancer cells are unclear. Here we show that elevated AURKC increases the proliferation, transformation and migration of cancer cells. Importantly, the kinase activity of AURKC is required for these tumour-associated properties. Analysis of human cancer specimens shows that the expression of AURKC is increased in cervical cancer, and is highly correlated with staging in colorectal cancer. Over-expressed AURKC-GFP localizes to the centromeric regions of mitotic chromosomes and results in a decreased level of AURKB, a key regulator of spindle checkpoint. Expression of AURKC is down-regulated by PLZF, a transcriptional repressor, through recruitment to its promoter region. The expression levels of PLZF and AURKC mRNA display opposite patterns in human cervical and colorectal cancers. Taken together, our results provide important insights into human cancers with AURKC expression, which may serve as a potential target for cancer therapy in the future.

Original languageEnglish
Pages (from-to)243-254
Number of pages12
JournalJournal of Pathology
Volume225
Issue number2
DOIs
Publication statusPublished - 2011 Oct 1

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Epithelial Cells
Neoplasms
Colorectal Neoplasms
Thyroid Neoplasms
Uterine Cervical Neoplasms
Genetic Promoter Regions
Cell Movement
Phosphotransferases
Chromosomes
Cell Line
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine

Cite this

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title = "Aberrantly expressed AURKC enhances the transformation and tumourigenicity of epithelial cells",
abstract = "Over-expression of AURKC has been detected in human colorectal cancers, thyroid carcinoma and several cancer cell lines. However, the regulation and clinical implications of over-expressed AURKC in cancer cells are unclear. Here we show that elevated AURKC increases the proliferation, transformation and migration of cancer cells. Importantly, the kinase activity of AURKC is required for these tumour-associated properties. Analysis of human cancer specimens shows that the expression of AURKC is increased in cervical cancer, and is highly correlated with staging in colorectal cancer. Over-expressed AURKC-GFP localizes to the centromeric regions of mitotic chromosomes and results in a decreased level of AURKB, a key regulator of spindle checkpoint. Expression of AURKC is down-regulated by PLZF, a transcriptional repressor, through recruitment to its promoter region. The expression levels of PLZF and AURKC mRNA display opposite patterns in human cervical and colorectal cancers. Taken together, our results provide important insights into human cancers with AURKC expression, which may serve as a potential target for cancer therapy in the future.",
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Aberrantly expressed AURKC enhances the transformation and tumourigenicity of epithelial cells. / Tsou, Jen Hui; Chang, Kung-Chao; Chang-Liao, Pey Yi; Yang, Shu Ting; Lee, Chung-Ta; Chen, Ya-Ping; Lee, Yi Chao; Lin, Po-Wen; Lee, Jenq-Chang; Shen, Meng-Ru; Chuang, Chin Kai; Chang, Wen Chang; Wang, Ju-Ming; Hung, Liang-Yi.

In: Journal of Pathology, Vol. 225, No. 2, 01.10.2011, p. 243-254.

Research output: Contribution to journalArticle

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AU - Tsou, Jen Hui

AU - Chang, Kung-Chao

AU - Chang-Liao, Pey Yi

AU - Yang, Shu Ting

AU - Lee, Chung-Ta

AU - Chen, Ya-Ping

AU - Lee, Yi Chao

AU - Lin, Po-Wen

AU - Lee, Jenq-Chang

AU - Shen, Meng-Ru

AU - Chuang, Chin Kai

AU - Chang, Wen Chang

AU - Wang, Ju-Ming

AU - Hung, Liang-Yi

PY - 2011/10/1

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N2 - Over-expression of AURKC has been detected in human colorectal cancers, thyroid carcinoma and several cancer cell lines. However, the regulation and clinical implications of over-expressed AURKC in cancer cells are unclear. Here we show that elevated AURKC increases the proliferation, transformation and migration of cancer cells. Importantly, the kinase activity of AURKC is required for these tumour-associated properties. Analysis of human cancer specimens shows that the expression of AURKC is increased in cervical cancer, and is highly correlated with staging in colorectal cancer. Over-expressed AURKC-GFP localizes to the centromeric regions of mitotic chromosomes and results in a decreased level of AURKB, a key regulator of spindle checkpoint. Expression of AURKC is down-regulated by PLZF, a transcriptional repressor, through recruitment to its promoter region. The expression levels of PLZF and AURKC mRNA display opposite patterns in human cervical and colorectal cancers. Taken together, our results provide important insights into human cancers with AURKC expression, which may serve as a potential target for cancer therapy in the future.

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