TY - JOUR
T1 - Ability of an alkali-tolerant mutant strain of the microalga Chlorella sp. AT1 to capture carbon dioxide for increasing carbon dioxide utilization efficiency
AU - Kuo, Chiu Mei
AU - Lin, Tsung Hsien
AU - Yang, Yi Chun
AU - Zhang, Wen Xin
AU - Lai, Jinn Tsyy
AU - Wu, Hsi Tien
AU - Chang, Jo Shu
AU - Lin, Chih Sheng
N1 - Funding Information:
The work was financially supported by the grants MOST 106-3113-E-006-011 and MOST 106-2621-M-009-001 from the Ministry of Science and Technology, Taiwan.
Publisher Copyright:
© 2017 Elsevier Ltd
Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2017
Y1 - 2017
N2 - An alkali-tolerant Chlorella sp. AT1 mutant strain was screened by NTG mutagenesis. The strain grew well in pH 6–11 media, and the optimal pH for growth was 10. The CO2 utilization efficiencies of Chlorella sp. AT1 cultured with intermittent 10% CO2 aeration for 10, 20 and 30 min at 3-h intervals were approximately 80, 42 and 30%, respectively. In alkaline medium (pH = 11) with intermittent 10% CO2 aeration for 30 min at 3-, 6- and 12-h intervals, the medium pH gradually changed to 10, and the biomass productivities of Chlorella sp. AT1 were 0.987, 0.848 and 0.710 g L−1 d−1, respectively. When Chlorella sp. AT1 was aerated with 10% CO2 intermittently for 30 min at 3-h intervals in semi-continuous cultivation for 21 days, the biomass concentration and biomass productivity were 4.35 g L−1 and 0.726 g L−1 d−1, respectively. Our results show that CO2 utilization efficiency can be markedly increased by intermittent CO2 aeration and alkaline media as a CO2-capturing strategy for alkali-tolerant microalga cultivation.
AB - An alkali-tolerant Chlorella sp. AT1 mutant strain was screened by NTG mutagenesis. The strain grew well in pH 6–11 media, and the optimal pH for growth was 10. The CO2 utilization efficiencies of Chlorella sp. AT1 cultured with intermittent 10% CO2 aeration for 10, 20 and 30 min at 3-h intervals were approximately 80, 42 and 30%, respectively. In alkaline medium (pH = 11) with intermittent 10% CO2 aeration for 30 min at 3-, 6- and 12-h intervals, the medium pH gradually changed to 10, and the biomass productivities of Chlorella sp. AT1 were 0.987, 0.848 and 0.710 g L−1 d−1, respectively. When Chlorella sp. AT1 was aerated with 10% CO2 intermittently for 30 min at 3-h intervals in semi-continuous cultivation for 21 days, the biomass concentration and biomass productivity were 4.35 g L−1 and 0.726 g L−1 d−1, respectively. Our results show that CO2 utilization efficiency can be markedly increased by intermittent CO2 aeration and alkaline media as a CO2-capturing strategy for alkali-tolerant microalga cultivation.
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U2 - 10.1016/j.biortech.2017.07.096
DO - 10.1016/j.biortech.2017.07.096
M3 - Article
C2 - 28780257
AN - SCOPUS:85026638348
SN - 0960-8524
VL - 244
SP - 243
EP - 251
JO - Bioresource technology
JF - Bioresource technology
ER -