Absence of CCR5-Δ32 mutation in healthy and HIV-1-infected aborigines in eastern Taiwan

Jaw Ming Cherng, Ji Hung Wang, Wen Lin Hsu, Kuo Liang Yang, Hsiao Yi Lin, Chrong-Reen Wang, Chi Mo Lin, Lih Shinn Wang

Research output: Contribution to journalArticle

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Abstract

Objective: Human immunodeficiency virus type 1 requires coreceptor CCR5 to enter into host cells. Homozygous deletion of 32 nucleotides (CCR5-Δ32) of CCR5 results in a severely truncated molecule and near-complete protection against HIV-1 infection. This deletion mutation is commonly found in Caucasians. However, no such a mutation has yet been found in Hong Kong and Taiwan. Furthermore, most current data in Taiwan were collected from non-aborigines. Therefore, we investigated whether CCR5Δ32 mutation occurs in aborigines in eastern Taiwan. Materials and Methods: Genomic DNA was extracted from healthy aborigines (n=1433) and HIV-1 infected aboriginal patients (n=11) in eastern Taiwan. Genotyping was performed using polymerase chain reaction (PCR) amplification of a portion of the CCR5 gene and analyzed with agarose gel electrophoresis. Results: All samples contained the wild-type CCR5 gene structure and no single deleted form of the CCR5 gene was found. Conclusion: Although some Taiwan aborigines are believed to have European and other ancestors as well as mainland Chinese ancestors, Taiwan aborigines have a CCR5 gene construction similar to non-aboriginal Taiwanese.

Original languageEnglish
Pages (from-to)353-357
Number of pages5
JournalTzu Chi Medical Journal
Volume14
Issue number6
Publication statusPublished - 2002 Dec 1

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Taiwan
HIV-1
Mutation
Genes
Agar Gel Electrophoresis
Sequence Deletion
Hong Kong
HIV Infections
Nucleotides
Polymerase Chain Reaction
DNA

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Cherng, J. M., Wang, J. H., Hsu, W. L., Yang, K. L., Lin, H. Y., Wang, C-R., ... Wang, L. S. (2002). Absence of CCR5-Δ32 mutation in healthy and HIV-1-infected aborigines in eastern Taiwan. Tzu Chi Medical Journal, 14(6), 353-357.
Cherng, Jaw Ming ; Wang, Ji Hung ; Hsu, Wen Lin ; Yang, Kuo Liang ; Lin, Hsiao Yi ; Wang, Chrong-Reen ; Lin, Chi Mo ; Wang, Lih Shinn. / Absence of CCR5-Δ32 mutation in healthy and HIV-1-infected aborigines in eastern Taiwan. In: Tzu Chi Medical Journal. 2002 ; Vol. 14, No. 6. pp. 353-357.
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abstract = "Objective: Human immunodeficiency virus type 1 requires coreceptor CCR5 to enter into host cells. Homozygous deletion of 32 nucleotides (CCR5-Δ32) of CCR5 results in a severely truncated molecule and near-complete protection against HIV-1 infection. This deletion mutation is commonly found in Caucasians. However, no such a mutation has yet been found in Hong Kong and Taiwan. Furthermore, most current data in Taiwan were collected from non-aborigines. Therefore, we investigated whether CCR5Δ32 mutation occurs in aborigines in eastern Taiwan. Materials and Methods: Genomic DNA was extracted from healthy aborigines (n=1433) and HIV-1 infected aboriginal patients (n=11) in eastern Taiwan. Genotyping was performed using polymerase chain reaction (PCR) amplification of a portion of the CCR5 gene and analyzed with agarose gel electrophoresis. Results: All samples contained the wild-type CCR5 gene structure and no single deleted form of the CCR5 gene was found. Conclusion: Although some Taiwan aborigines are believed to have European and other ancestors as well as mainland Chinese ancestors, Taiwan aborigines have a CCR5 gene construction similar to non-aboriginal Taiwanese.",
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Cherng, JM, Wang, JH, Hsu, WL, Yang, KL, Lin, HY, Wang, C-R, Lin, CM & Wang, LS 2002, 'Absence of CCR5-Δ32 mutation in healthy and HIV-1-infected aborigines in eastern Taiwan', Tzu Chi Medical Journal, vol. 14, no. 6, pp. 353-357.

Absence of CCR5-Δ32 mutation in healthy and HIV-1-infected aborigines in eastern Taiwan. / Cherng, Jaw Ming; Wang, Ji Hung; Hsu, Wen Lin; Yang, Kuo Liang; Lin, Hsiao Yi; Wang, Chrong-Reen; Lin, Chi Mo; Wang, Lih Shinn.

In: Tzu Chi Medical Journal, Vol. 14, No. 6, 01.12.2002, p. 353-357.

Research output: Contribution to journalArticle

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AU - Cherng, Jaw Ming

AU - Wang, Ji Hung

AU - Hsu, Wen Lin

AU - Yang, Kuo Liang

AU - Lin, Hsiao Yi

AU - Wang, Chrong-Reen

AU - Lin, Chi Mo

AU - Wang, Lih Shinn

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N2 - Objective: Human immunodeficiency virus type 1 requires coreceptor CCR5 to enter into host cells. Homozygous deletion of 32 nucleotides (CCR5-Δ32) of CCR5 results in a severely truncated molecule and near-complete protection against HIV-1 infection. This deletion mutation is commonly found in Caucasians. However, no such a mutation has yet been found in Hong Kong and Taiwan. Furthermore, most current data in Taiwan were collected from non-aborigines. Therefore, we investigated whether CCR5Δ32 mutation occurs in aborigines in eastern Taiwan. Materials and Methods: Genomic DNA was extracted from healthy aborigines (n=1433) and HIV-1 infected aboriginal patients (n=11) in eastern Taiwan. Genotyping was performed using polymerase chain reaction (PCR) amplification of a portion of the CCR5 gene and analyzed with agarose gel electrophoresis. Results: All samples contained the wild-type CCR5 gene structure and no single deleted form of the CCR5 gene was found. Conclusion: Although some Taiwan aborigines are believed to have European and other ancestors as well as mainland Chinese ancestors, Taiwan aborigines have a CCR5 gene construction similar to non-aboriginal Taiwanese.

AB - Objective: Human immunodeficiency virus type 1 requires coreceptor CCR5 to enter into host cells. Homozygous deletion of 32 nucleotides (CCR5-Δ32) of CCR5 results in a severely truncated molecule and near-complete protection against HIV-1 infection. This deletion mutation is commonly found in Caucasians. However, no such a mutation has yet been found in Hong Kong and Taiwan. Furthermore, most current data in Taiwan were collected from non-aborigines. Therefore, we investigated whether CCR5Δ32 mutation occurs in aborigines in eastern Taiwan. Materials and Methods: Genomic DNA was extracted from healthy aborigines (n=1433) and HIV-1 infected aboriginal patients (n=11) in eastern Taiwan. Genotyping was performed using polymerase chain reaction (PCR) amplification of a portion of the CCR5 gene and analyzed with agarose gel electrophoresis. Results: All samples contained the wild-type CCR5 gene structure and no single deleted form of the CCR5 gene was found. Conclusion: Although some Taiwan aborigines are believed to have European and other ancestors as well as mainland Chinese ancestors, Taiwan aborigines have a CCR5 gene construction similar to non-aboriginal Taiwanese.

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