Activation of the ubiquitin proteasome pathway by silk fibroin modified chitosan nanoparticles in hepatic cancer cells

Ming Hui Yang, Tze Wen Chung, Yi Shan Lu, Yi Ling Chen, Wan Chi Tsai, Shiang Bin Jong, Shyng Shiou Yuan, Pao Chi Liao, Po Chiao Lin, Yu Chang Tyan

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7 Citations (Scopus)

Abstract

Silk fibroin (SF) is a protein with bulky hydrophobic domains and can be easily purified as sericin-free silk-based biomaterial. Silk fibroin modified chitosan nanoparticle (SF-CSNP), a biocompatible material, has been widely used as a potential drug delivery system. Our current investigation studied the bio-effects of the SF-CSNP uptake by liver cells. In this experiment, the characterizations of SF-CSNPs were measured by particle size analysis and protein assay. The average size of the SF-CSNP was 311.9 ± 10.7 nm, and the average zeta potential was +13.33 ± 0.3 mV. The SF coating on the SF-CSNP was 6.27 ± 0.17 μg/mL. Moreover, using proteomic approaches, several proteins involved in the ubiquitin proteasome pathway were identified by analysis of differential protein expressions of HepG2 cell uptake the SF-CSNP. Our experimental results have demonstrated that the SF-CSNP may be involved in liver cancer cell survival and proliferation.

Original languageEnglish
Pages (from-to)1657-1676
Number of pages20
JournalInternational journal of molecular sciences
Volume16
Issue number1
DOIs
Publication statusPublished - 2015 Jan 12

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

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    Yang, M. H., Chung, T. W., Lu, Y. S., Chen, Y. L., Tsai, W. C., Jong, S. B., Yuan, S. S., Liao, P. C., Lin, P. C., & Tyan, Y. C. (2015). Activation of the ubiquitin proteasome pathway by silk fibroin modified chitosan nanoparticles in hepatic cancer cells. International journal of molecular sciences, 16(1), 1657-1676. https://doi.org/10.3390/ijms16011657