Acutely administered melatonin reduces oxidative damage in lung and brain induced by hyperbaric oxygen

M. I. Pablos, R. J. Reiter, Jih-Ing Chuang, G. G. Ortiz, J. M. Guerrero, E. Sewerynek, M. T. Agapito, D. Melchiorri, R. Lawrence, S. M. Deneke

Research output: Contribution to journalArticle

96 Citations (Scopus)

Abstract

Hyperbaric oxygen exposure rapidly induces lipid peroxidation and cellular damage in a variety of organs. In this study, we demonstrate that the exposure of rats to 4 atmospheres of 100% oxygen for 90 min is associated with increased levels of lipid peroxidation products [malonaldehyde (MDA) and 4-hydroxyalkenals (4-HDA)] and with changes in the activities of two antioxidative enzymes [glutathione peroxidase (GPX) and glutathione reductase (GR)], as well as in the glutathione status in the lungs and in the brain. Products of lipid peroxidation increased after hyperbaric hyperoxia, both GPX and GR activities were decreased, and levels of total glutathione (reduced+oxidized) and glutathione disulfide (oxidized glutathione) increased in both lung and brain areas (cerebral cortex, hippocampus, hypothalamus, striatum, and cerebellum) but not in liver. When animals were injected with melatonin (10 mg/kg) immediately before the 90-min hyperbaric oxygen exposure, all measurements of oxidative damage were prevented and were similar to those in untreated control animals. Melatonin's actions may be related to a variety of mechanisms, some of which remain to be identified, including its ability to directly scavenge free radicals and its induction of antioxidative enzymes via specific melatonin receptors.

Original languageEnglish
Pages (from-to)354-358
Number of pages5
JournalJournal of Applied Physiology
Volume83
Issue number2
Publication statusPublished - 1997 Aug 20

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Glutathione Disulfide
Melatonin
Lipid Peroxidation
Glutathione Reductase
Oxygen
Glutathione Peroxidase
Lung
Glutathione
Brain
Melatonin Receptors
Hyperoxia
Enzyme Induction
Malondialdehyde
Atmosphere
Cerebral Cortex
Cerebellum
Hypothalamus
Free Radicals
Hippocampus
Liver

All Science Journal Classification (ASJC) codes

  • Physiology
  • Physiology (medical)

Cite this

Pablos, M. I., Reiter, R. J., Chuang, J-I., Ortiz, G. G., Guerrero, J. M., Sewerynek, E., ... Deneke, S. M. (1997). Acutely administered melatonin reduces oxidative damage in lung and brain induced by hyperbaric oxygen. Journal of Applied Physiology, 83(2), 354-358.
Pablos, M. I. ; Reiter, R. J. ; Chuang, Jih-Ing ; Ortiz, G. G. ; Guerrero, J. M. ; Sewerynek, E. ; Agapito, M. T. ; Melchiorri, D. ; Lawrence, R. ; Deneke, S. M. / Acutely administered melatonin reduces oxidative damage in lung and brain induced by hyperbaric oxygen. In: Journal of Applied Physiology. 1997 ; Vol. 83, No. 2. pp. 354-358.
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abstract = "Hyperbaric oxygen exposure rapidly induces lipid peroxidation and cellular damage in a variety of organs. In this study, we demonstrate that the exposure of rats to 4 atmospheres of 100{\%} oxygen for 90 min is associated with increased levels of lipid peroxidation products [malonaldehyde (MDA) and 4-hydroxyalkenals (4-HDA)] and with changes in the activities of two antioxidative enzymes [glutathione peroxidase (GPX) and glutathione reductase (GR)], as well as in the glutathione status in the lungs and in the brain. Products of lipid peroxidation increased after hyperbaric hyperoxia, both GPX and GR activities were decreased, and levels of total glutathione (reduced+oxidized) and glutathione disulfide (oxidized glutathione) increased in both lung and brain areas (cerebral cortex, hippocampus, hypothalamus, striatum, and cerebellum) but not in liver. When animals were injected with melatonin (10 mg/kg) immediately before the 90-min hyperbaric oxygen exposure, all measurements of oxidative damage were prevented and were similar to those in untreated control animals. Melatonin's actions may be related to a variety of mechanisms, some of which remain to be identified, including its ability to directly scavenge free radicals and its induction of antioxidative enzymes via specific melatonin receptors.",
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Pablos, MI, Reiter, RJ, Chuang, J-I, Ortiz, GG, Guerrero, JM, Sewerynek, E, Agapito, MT, Melchiorri, D, Lawrence, R & Deneke, SM 1997, 'Acutely administered melatonin reduces oxidative damage in lung and brain induced by hyperbaric oxygen', Journal of Applied Physiology, vol. 83, no. 2, pp. 354-358.

Acutely administered melatonin reduces oxidative damage in lung and brain induced by hyperbaric oxygen. / Pablos, M. I.; Reiter, R. J.; Chuang, Jih-Ing; Ortiz, G. G.; Guerrero, J. M.; Sewerynek, E.; Agapito, M. T.; Melchiorri, D.; Lawrence, R.; Deneke, S. M.

In: Journal of Applied Physiology, Vol. 83, No. 2, 20.08.1997, p. 354-358.

Research output: Contribution to journalArticle

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