Adenosine Stimulates Human Sperm Motility via A2 Receptors

MENG‐RU ‐R SHEN, JOEL LINDEN, PO‐HUI ‐H CHIANG, SHUN‐SHENG ‐S CHEN, SHENG‐NAN ‐N WU

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Abstract

Abstract— The effects of adenosine and its analogues on human sperm motility were studied using a transmembrane migration method. Specific binding sites for adenosine in human sperm were also investigated. Adenosine and 5′‐N‐ethylcarboxamidoadenosine (NECA) stimulated human sperm motility with similar efficacies and the maximal amplitudes of motility increases were both about 70%. 3,7‐Dimethyl‐1‐propargylxanthine (DMPX), a potent A2 antagonist, competitively antagonized NECA‐induced motility stimulation. Successively higher concentrations of DMPX shifted the dose‐response curve of NECA to the right in a nearly parallel fashion. Dipyridamole, an inhibitor of adenosine uptake, does not reduce the ability of adenosine to stimulate human sperm motility. In radioligand‐binding studies, adenosine A1 selective analogues, cyclopentyl‐1,3‐dipropylxanthine and 1‐methyl‐2‐phenylethyl adenosine, have little competitive effect on [3H]NECA binding in human sperm membrane. These results provide evidence that adenosine enhances human sperm motility via adenosine A2 receptors on the surface of sperm membranes. 1993 Royal Pharmaceutical Society of Great Britain

Original languageEnglish
Pages (from-to)650-653
Number of pages4
JournalJournal of Pharmacy and Pharmacology
Volume45
Issue number7
DOIs
Publication statusPublished - 1993 Jul

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmaceutical Science

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