TY - JOUR
T1 - Advanced applications of next-generation sequencing technologies to orchid biology
AU - Yeh, Chuan Ming
AU - Liu, Zhong Jian
AU - Tsai, Wen Chieh
N1 - Funding Information:
This work was supported by Grants 103-2313-B-006-001-MY3, 104-2321-B-006-025-, and 105-2321-B-006-026-from Ministry of Science and Technology, Taiwan, and was supported by the 948 Program of State Forestry Administration, China (No. 2011-4-53), Development Special Fund of Biological Industry of Shenzhen Municipality (No. JC201005310692A), Development Funds for Emerging Industries of Strategic Importance of Shenzhen (No. JCYJ20140402093332029, No. NYSW20140331010039), Fundamental Research Project of Shenzhen Municipality (No. JCYJ20150403150235943), and Forestry Science and Technology Innovation Fund Project of Guangdong Province (No. 2016KJCX025).
Publisher Copyright:
© 2018, Caister Academic Press. All rights reserved.
PY - 2018
Y1 - 2018
N2 - Next-generation sequencing (NGS) technologies are revolutionizing biology by permitting transcriptome sequencing, whole-genome sequencing and resequencing, and genome-wide single nucleotide polymorphism profiling. Orchid research has benefited from this breakthrough, and a few orchid genomes are now available; new biological questions can be approached and new breeding strategies can be designed. The first part of this review describes the unique features of orchid biology. The second part provides an overview of the current NGS platforms, many of which are already used in plant laboratories. The third part summarizes the state of orchid transcriptome and genome sequencing and illustrates current achievements. The genetic sequences currently obtained will not only provide a broad scope for the study of orchid biology, but also serves as a starting point for uncovering the mystery of orchid evolution.
AB - Next-generation sequencing (NGS) technologies are revolutionizing biology by permitting transcriptome sequencing, whole-genome sequencing and resequencing, and genome-wide single nucleotide polymorphism profiling. Orchid research has benefited from this breakthrough, and a few orchid genomes are now available; new biological questions can be approached and new breeding strategies can be designed. The first part of this review describes the unique features of orchid biology. The second part provides an overview of the current NGS platforms, many of which are already used in plant laboratories. The third part summarizes the state of orchid transcriptome and genome sequencing and illustrates current achievements. The genetic sequences currently obtained will not only provide a broad scope for the study of orchid biology, but also serves as a starting point for uncovering the mystery of orchid evolution.
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U2 - 10.21775/cimb.027.051
DO - 10.21775/cimb.027.051
M3 - Article
C2 - 28885174
AN - SCOPUS:85051181088
SN - 1467-3037
VL - 27
SP - 51
EP - 69
JO - Current issues in molecular biology
JF - Current issues in molecular biology
ER -