Advanced glycation end products-mediated hypertrophy is negatively regulated by tetrahydrobiopterin in renal tubular cells

Yi Chen Liao, Ying Ho Lee, Lea Yea Chuang, Jinn Yuh Guh, Ming Der Shi, Jau Shyang Huang

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

Diabetic nephropathy (DN) is the most common cause of end-stage renal disease worldwide. The accumulation of advanced glycation end products (AGE) is a key mediator of renal tubular hypertrophy in DN. Elimination of tetrahydrobiopterin (BH 4) and nitric oxide (NO) bioavailability may contribute to the aggravation of DN. The present study aims to explore any possible beneficial effect of exogenous BH 4 in alleviating the AGE-induced renal tubular hypertrophy in DN. Thus, renal tubular cells were treated with BH 4, BH 2, sepiapterin, or DAHP in the presence of AGE. We found that AGE (but not non-glycated BSA) markedly reduced NO production and increased hypertrophy index in these cells. Exogenous BH 4/BH 2 and sepiapterin treatments attenuated AGE-inhibited the iNOS/NO/GTPCH I protein synthesis. Moreover, BH 4 and BH 2 significantly reversed AGE-enhanced the JAK2-STAT1/STAT3 activation. The abilities of BH 4 and BH 2 to inhibit AGE-induced renal cellular hypertrophy were verified by the observation that BH 4 and BH 2 inhibited hypertrophic growth and the protein synthesis of p27 Kip1 and α-SMA. These findings indicate for the first time that exogenous BH 4 and BH 2 attenuate AGE-induced hypertrophic effect at least partly by increasing the iNOS/GTPCH I synthesis and NO generation in renal tubular cells.

Original languageEnglish
Pages (from-to)71-77
Number of pages7
JournalMolecular and Cellular Endocrinology
Volume355
Issue number1
DOIs
Publication statusPublished - 2012 May 15

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Endocrinology

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