Advances in nonlinear optical microscopy for visualizing dynamic tissue properties in culture

Alvin T. Yeh, Holly Gibbs, Jin Jia Hu, Adam M. Larson

Research output: Contribution to journalReview articlepeer-review

17 Citations (Scopus)


Optical microscopy encompasses high-resolution imaging techniques that can be used to non-destructively investigate and characterize living biological systems and engineered tissue constructs in culture. In particular, nonlinear optical microscopy (NLOM) is well suited for the visualization and quantification of processes involved in cell-extracellular matrix interactions in vivo. Current NLOM technology enables concomitant molecular imaging and visualization of microstructural organization that could provide a direct link between signal transduction and biological effect at microscopic length scales that culminate into tissue macroscopic properties and function. This review highlights the fundamentals of nonlinear optical interactions between light and tissue and presents a direction for future technology development to better complement quantitative, high-throughput assays of the modern life sciences.

Original languageEnglish
Pages (from-to)119-131
Number of pages13
JournalTissue Engineering - Part B: Reviews
Issue number1
Publication statusPublished - 2008 Mar 1

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biomaterials
  • Biochemistry
  • Biomedical Engineering


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