Aeromonas stool isolates from individuals with or without diarrhea in southern Taiwan: Predominance of Aeromonas veronii

Po Lin Chen, Pei Jane Tsai, Chang Shi Chen, Ying Chuan Lu, Hung Mo Chen, Nan Yao Lee, Ching Chi Lee, Chia Wen Li, Ming Chi Li, Chi Jung Wu, Wen Chien Ko

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10 Citations (Scopus)

Abstract

Background: Although aeromonads are important pathogens causing invasive infections in southern Taiwan, Aeromonas-associated intestinal infections have been rarely mentioned. Purpose: The aim of this study was to understand the frequency of isolation and clinical significance of aeromonads recovered from adult stool samples in southern Taiwan. Methods: During a 15-month study period, 514 adults with diarrhea and 167 asymptomatic controls were prospectively screened for the presence of aeromonads in stools. The identity of Aeromonas species was determined by the rpoD sequencing. Clinical information was retrieved from medical records, and in vitro cytotoxicity assay and polymerase chain reaction detection of putative virulent genes were performed. Results: Thirteen (2.5 %) of 514 diarrheal patients and six (3.6%) of 167 asymptomatic controls had Aeromonas isolates in their stools. Of 11 diarrheal patients with available clinical information, Aeromonas veronii, the predominant species, was noted in six patients, and another potential enteropathogen was present in four patients. The cytotoxicity of A. veronii isolates to the HT-29 cell line was more potent in the isolates from diarrheal patients than those from asymptomatic controls (p = 0.015). The cytotoxicity of A. veronii isolates was more potent than that of A. caviae from symptomatic patients (p = 0.001). Putative virulence markers, including AHCYTONE, ascV, ascF-ascG, and aexT, were detected exclusively in A. veronii. The presence of the ascV gene was associated with cytotoxicity in A. veronii isolates. All Aeromonas isolates were susceptible to varied antimicrobial agents, except ampicillin/sulbactam. Conclusion: A. veronii is the predominant species in stools from individuals with or without diarrhea in southern Taiwan.

Original languageEnglish
Pages (from-to)618-624
Number of pages7
JournalJournal of Microbiology, Immunology and Infection
Volume48
Issue number6
DOIs
Publication statusPublished - 2015 Dec 1

Fingerprint

Aeromonas
Taiwan
Diarrhea
HT29 Cells
Anti-Infective Agents
Infection
Genes
Medical Records
Virulence
Aeromonas veronii
Cell Line
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology and Microbiology(all)
  • Microbiology (medical)
  • Infectious Diseases

Cite this

@article{3b171ee399784b8aade8af65864cf287,
title = "Aeromonas stool isolates from individuals with or without diarrhea in southern Taiwan: Predominance of Aeromonas veronii",
abstract = "Background: Although aeromonads are important pathogens causing invasive infections in southern Taiwan, Aeromonas-associated intestinal infections have been rarely mentioned. Purpose: The aim of this study was to understand the frequency of isolation and clinical significance of aeromonads recovered from adult stool samples in southern Taiwan. Methods: During a 15-month study period, 514 adults with diarrhea and 167 asymptomatic controls were prospectively screened for the presence of aeromonads in stools. The identity of Aeromonas species was determined by the rpoD sequencing. Clinical information was retrieved from medical records, and in vitro cytotoxicity assay and polymerase chain reaction detection of putative virulent genes were performed. Results: Thirteen (2.5 {\%}) of 514 diarrheal patients and six (3.6{\%}) of 167 asymptomatic controls had Aeromonas isolates in their stools. Of 11 diarrheal patients with available clinical information, Aeromonas veronii, the predominant species, was noted in six patients, and another potential enteropathogen was present in four patients. The cytotoxicity of A. veronii isolates to the HT-29 cell line was more potent in the isolates from diarrheal patients than those from asymptomatic controls (p = 0.015). The cytotoxicity of A. veronii isolates was more potent than that of A. caviae from symptomatic patients (p = 0.001). Putative virulence markers, including AHCYTONE, ascV, ascF-ascG, and aexT, were detected exclusively in A. veronii. The presence of the ascV gene was associated with cytotoxicity in A. veronii isolates. All Aeromonas isolates were susceptible to varied antimicrobial agents, except ampicillin/sulbactam. Conclusion: A. veronii is the predominant species in stools from individuals with or without diarrhea in southern Taiwan.",
author = "Chen, {Po Lin} and Tsai, {Pei Jane} and Chen, {Chang Shi} and Lu, {Ying Chuan} and Chen, {Hung Mo} and Lee, {Nan Yao} and Lee, {Ching Chi} and Li, {Chia Wen} and Li, {Ming Chi} and Wu, {Chi Jung} and Ko, {Wen Chien}",
year = "2015",
month = "12",
day = "1",
doi = "10.1016/j.jmii.2014.08.007",
language = "English",
volume = "48",
pages = "618--624",
journal = "Journal of Microbiology, Immunology and Infection",
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publisher = "Elsevier Taiwan LLC",
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}

TY - JOUR

T1 - Aeromonas stool isolates from individuals with or without diarrhea in southern Taiwan

T2 - Predominance of Aeromonas veronii

AU - Chen, Po Lin

AU - Tsai, Pei Jane

AU - Chen, Chang Shi

AU - Lu, Ying Chuan

AU - Chen, Hung Mo

AU - Lee, Nan Yao

AU - Lee, Ching Chi

AU - Li, Chia Wen

AU - Li, Ming Chi

AU - Wu, Chi Jung

AU - Ko, Wen Chien

PY - 2015/12/1

Y1 - 2015/12/1

N2 - Background: Although aeromonads are important pathogens causing invasive infections in southern Taiwan, Aeromonas-associated intestinal infections have been rarely mentioned. Purpose: The aim of this study was to understand the frequency of isolation and clinical significance of aeromonads recovered from adult stool samples in southern Taiwan. Methods: During a 15-month study period, 514 adults with diarrhea and 167 asymptomatic controls were prospectively screened for the presence of aeromonads in stools. The identity of Aeromonas species was determined by the rpoD sequencing. Clinical information was retrieved from medical records, and in vitro cytotoxicity assay and polymerase chain reaction detection of putative virulent genes were performed. Results: Thirteen (2.5 %) of 514 diarrheal patients and six (3.6%) of 167 asymptomatic controls had Aeromonas isolates in their stools. Of 11 diarrheal patients with available clinical information, Aeromonas veronii, the predominant species, was noted in six patients, and another potential enteropathogen was present in four patients. The cytotoxicity of A. veronii isolates to the HT-29 cell line was more potent in the isolates from diarrheal patients than those from asymptomatic controls (p = 0.015). The cytotoxicity of A. veronii isolates was more potent than that of A. caviae from symptomatic patients (p = 0.001). Putative virulence markers, including AHCYTONE, ascV, ascF-ascG, and aexT, were detected exclusively in A. veronii. The presence of the ascV gene was associated with cytotoxicity in A. veronii isolates. All Aeromonas isolates were susceptible to varied antimicrobial agents, except ampicillin/sulbactam. Conclusion: A. veronii is the predominant species in stools from individuals with or without diarrhea in southern Taiwan.

AB - Background: Although aeromonads are important pathogens causing invasive infections in southern Taiwan, Aeromonas-associated intestinal infections have been rarely mentioned. Purpose: The aim of this study was to understand the frequency of isolation and clinical significance of aeromonads recovered from adult stool samples in southern Taiwan. Methods: During a 15-month study period, 514 adults with diarrhea and 167 asymptomatic controls were prospectively screened for the presence of aeromonads in stools. The identity of Aeromonas species was determined by the rpoD sequencing. Clinical information was retrieved from medical records, and in vitro cytotoxicity assay and polymerase chain reaction detection of putative virulent genes were performed. Results: Thirteen (2.5 %) of 514 diarrheal patients and six (3.6%) of 167 asymptomatic controls had Aeromonas isolates in their stools. Of 11 diarrheal patients with available clinical information, Aeromonas veronii, the predominant species, was noted in six patients, and another potential enteropathogen was present in four patients. The cytotoxicity of A. veronii isolates to the HT-29 cell line was more potent in the isolates from diarrheal patients than those from asymptomatic controls (p = 0.015). The cytotoxicity of A. veronii isolates was more potent than that of A. caviae from symptomatic patients (p = 0.001). Putative virulence markers, including AHCYTONE, ascV, ascF-ascG, and aexT, were detected exclusively in A. veronii. The presence of the ascV gene was associated with cytotoxicity in A. veronii isolates. All Aeromonas isolates were susceptible to varied antimicrobial agents, except ampicillin/sulbactam. Conclusion: A. veronii is the predominant species in stools from individuals with or without diarrhea in southern Taiwan.

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U2 - 10.1016/j.jmii.2014.08.007

DO - 10.1016/j.jmii.2014.08.007

M3 - Article

C2 - 25440979

AN - SCOPUS:84952876068

VL - 48

SP - 618

EP - 624

JO - Journal of Microbiology, Immunology and Infection

JF - Journal of Microbiology, Immunology and Infection

SN - 1684-1182

IS - 6

ER -