Affinity capture using vancomycin-bound magnetic nanoparticles for the MALDI-MS analysis of bacteria

Ya Shiuan Lin, Pei Jane Tsai, Mao Feng Weng, Yu Chie Chen

Research output: Contribution to journalArticle

183 Citations (Scopus)

Abstract

Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) provides a straightforward means to differentiate microorganism species based on mass spectral fingerprinting. The pathogen cell concentration in an infected sample, however, is generally lower than that capable of being detected directly by MALDI-MS. Furthermore, the presence of proteins or metabolites in biological fluids always causes unavoidable interference for the identification of microorganism species. Vancomycin, which binds to D-Ala-D-Ala moieties on the cell walls of Gram-positive bacteria and, therefore, inhibits peptidoglycan synthesis, is one of the most potent antibiotics. Thus, we have employed vancomycin-modified magnetic nanoparticles as affinity probes to selectively trap Gram-positive pathogens from sample solutions; i.e., these bacteria can be isolated from sample solutions by applying a magnetic field. The isolated cells could then be characterized by MALDI-MS. This approach effectively reduces the interference of protein and metabolite signals in the mass spectra of Gram-positive bacteria because vancomycin has such high specificity for the D-Ala-D-Ala units of the cell walls. The lowest cell concentration we detected for both Staphylococcus saprophyticus and Staphylococcus aureus in a urine sample (3 mL) was ∼7 × 104 cfu/mL.

Original languageEnglish
Pages (from-to)1753-1760
Number of pages8
JournalAnalytical chemistry
Volume77
Issue number6
DOIs
Publication statusPublished - 2005 Mar 15

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

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