Agaricus blazei Murill ameliorates myocardial ischemia-reperfusion injury

Chien Hua Huang, Han Ni Tsai, Ren How Peng, Yi Hsuan Hsieh, Woei-Jer Chuang, Ga Ching Hsu, Wen-Ya Huang, Wen Jone Chen

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Background: Agaricus blazei Murill (ABM) is a widely accepted health food and is known to present anti-tumorigenic activities. ABM has also been reported to modulate oxidative stress after inflammation and infection. Reactive oxygen free radicals have been shown to be critically involved in ischemia-reperfusion injury cascades. We aimed to study the antioxidant activities of ABM against tissue injury caused by myocardial ischemia- reperfusion processes. Methods: A rat cardiomyocyte H9c2 cell line was set up and used for the study. Cell viabilities after different dosages of ABM extract treatment and hydrogen peroxide injuries were checked. A cardiac ischemia-reperfusion injury model was set up to elucidate the cardioprotective roles of ABM extract. Different doses of 22.5 mg/kg of body weight and 45 mg/kg of body weight ABM extract were pre-treated at 24 and 48 hours before inducing the ischemia-reperfusion injury. Results: ABM extract treatment increased the survival of cardiomyocytes without cytotoxicity. Pretreatment with ABM extract reduced hydrogen peroxide-induced cell damage and increased cell survival (0.86 ± 0.15 in ABM 6.0 mg/ml vs. 0.45 ± 0.03 in control, p = 0.019). ABM-pretreated rats that underwent myocardial ischemia-reperfusion had greatly reduced infarct areas compared to those in the control group (14.3 ± 3.3% in control group, 4.6 ± 1.9% in 22.5 mg/kg group, 4.9 ± 2.1% in 45 mg/kg group, p < 0.05). Conclusion: ABM increases antioxidant activity, which greatly ameliorates myocardial injuries caused by myocardial ischemia-reperfusion injuries. Using ABM as a health food may provide cardioprotective effect against ischemia-reperfusion injuries.

Original languageEnglish
Pages (from-to)235-241
Number of pages7
JournalActa Cardiologica Sinica
Volume26
Issue number4
Publication statusPublished - 2010 Dec 1

Fingerprint

Agaricus
Myocardial Reperfusion Injury
Reperfusion Injury
Myocardial Ischemia
Myocardial Reperfusion
Cardiac Myocytes
Hydrogen Peroxide
Cell Survival
Wounds and Injuries
Antioxidants
Body Weight
Food
Control Groups
Health
Free Radicals
Reactive Oxygen Species

All Science Journal Classification (ASJC) codes

  • Cardiology and Cardiovascular Medicine

Cite this

Huang, C. H., Tsai, H. N., Peng, R. H., Hsieh, Y. H., Chuang, W-J., Hsu, G. C., ... Chen, W. J. (2010). Agaricus blazei Murill ameliorates myocardial ischemia-reperfusion injury. Acta Cardiologica Sinica, 26(4), 235-241.
Huang, Chien Hua ; Tsai, Han Ni ; Peng, Ren How ; Hsieh, Yi Hsuan ; Chuang, Woei-Jer ; Hsu, Ga Ching ; Huang, Wen-Ya ; Chen, Wen Jone. / Agaricus blazei Murill ameliorates myocardial ischemia-reperfusion injury. In: Acta Cardiologica Sinica. 2010 ; Vol. 26, No. 4. pp. 235-241.
@article{d0771b6d96ff41a7ba7512d8dc4da351,
title = "Agaricus blazei Murill ameliorates myocardial ischemia-reperfusion injury",
abstract = "Background: Agaricus blazei Murill (ABM) is a widely accepted health food and is known to present anti-tumorigenic activities. ABM has also been reported to modulate oxidative stress after inflammation and infection. Reactive oxygen free radicals have been shown to be critically involved in ischemia-reperfusion injury cascades. We aimed to study the antioxidant activities of ABM against tissue injury caused by myocardial ischemia- reperfusion processes. Methods: A rat cardiomyocyte H9c2 cell line was set up and used for the study. Cell viabilities after different dosages of ABM extract treatment and hydrogen peroxide injuries were checked. A cardiac ischemia-reperfusion injury model was set up to elucidate the cardioprotective roles of ABM extract. Different doses of 22.5 mg/kg of body weight and 45 mg/kg of body weight ABM extract were pre-treated at 24 and 48 hours before inducing the ischemia-reperfusion injury. Results: ABM extract treatment increased the survival of cardiomyocytes without cytotoxicity. Pretreatment with ABM extract reduced hydrogen peroxide-induced cell damage and increased cell survival (0.86 ± 0.15 in ABM 6.0 mg/ml vs. 0.45 ± 0.03 in control, p = 0.019). ABM-pretreated rats that underwent myocardial ischemia-reperfusion had greatly reduced infarct areas compared to those in the control group (14.3 ± 3.3{\%} in control group, 4.6 ± 1.9{\%} in 22.5 mg/kg group, 4.9 ± 2.1{\%} in 45 mg/kg group, p < 0.05). Conclusion: ABM increases antioxidant activity, which greatly ameliorates myocardial injuries caused by myocardial ischemia-reperfusion injuries. Using ABM as a health food may provide cardioprotective effect against ischemia-reperfusion injuries.",
author = "Huang, {Chien Hua} and Tsai, {Han Ni} and Peng, {Ren How} and Hsieh, {Yi Hsuan} and Woei-Jer Chuang and Hsu, {Ga Ching} and Wen-Ya Huang and Chen, {Wen Jone}",
year = "2010",
month = "12",
day = "1",
language = "English",
volume = "26",
pages = "235--241",
journal = "Acta Cardiologica Sinica",
issn = "1011-6842",
publisher = "Republic of China Society of Cardiology",
number = "4",

}

Huang, CH, Tsai, HN, Peng, RH, Hsieh, YH, Chuang, W-J, Hsu, GC, Huang, W-Y & Chen, WJ 2010, 'Agaricus blazei Murill ameliorates myocardial ischemia-reperfusion injury', Acta Cardiologica Sinica, vol. 26, no. 4, pp. 235-241.

Agaricus blazei Murill ameliorates myocardial ischemia-reperfusion injury. / Huang, Chien Hua; Tsai, Han Ni; Peng, Ren How; Hsieh, Yi Hsuan; Chuang, Woei-Jer; Hsu, Ga Ching; Huang, Wen-Ya; Chen, Wen Jone.

In: Acta Cardiologica Sinica, Vol. 26, No. 4, 01.12.2010, p. 235-241.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Agaricus blazei Murill ameliorates myocardial ischemia-reperfusion injury

AU - Huang, Chien Hua

AU - Tsai, Han Ni

AU - Peng, Ren How

AU - Hsieh, Yi Hsuan

AU - Chuang, Woei-Jer

AU - Hsu, Ga Ching

AU - Huang, Wen-Ya

AU - Chen, Wen Jone

PY - 2010/12/1

Y1 - 2010/12/1

N2 - Background: Agaricus blazei Murill (ABM) is a widely accepted health food and is known to present anti-tumorigenic activities. ABM has also been reported to modulate oxidative stress after inflammation and infection. Reactive oxygen free radicals have been shown to be critically involved in ischemia-reperfusion injury cascades. We aimed to study the antioxidant activities of ABM against tissue injury caused by myocardial ischemia- reperfusion processes. Methods: A rat cardiomyocyte H9c2 cell line was set up and used for the study. Cell viabilities after different dosages of ABM extract treatment and hydrogen peroxide injuries were checked. A cardiac ischemia-reperfusion injury model was set up to elucidate the cardioprotective roles of ABM extract. Different doses of 22.5 mg/kg of body weight and 45 mg/kg of body weight ABM extract were pre-treated at 24 and 48 hours before inducing the ischemia-reperfusion injury. Results: ABM extract treatment increased the survival of cardiomyocytes without cytotoxicity. Pretreatment with ABM extract reduced hydrogen peroxide-induced cell damage and increased cell survival (0.86 ± 0.15 in ABM 6.0 mg/ml vs. 0.45 ± 0.03 in control, p = 0.019). ABM-pretreated rats that underwent myocardial ischemia-reperfusion had greatly reduced infarct areas compared to those in the control group (14.3 ± 3.3% in control group, 4.6 ± 1.9% in 22.5 mg/kg group, 4.9 ± 2.1% in 45 mg/kg group, p < 0.05). Conclusion: ABM increases antioxidant activity, which greatly ameliorates myocardial injuries caused by myocardial ischemia-reperfusion injuries. Using ABM as a health food may provide cardioprotective effect against ischemia-reperfusion injuries.

AB - Background: Agaricus blazei Murill (ABM) is a widely accepted health food and is known to present anti-tumorigenic activities. ABM has also been reported to modulate oxidative stress after inflammation and infection. Reactive oxygen free radicals have been shown to be critically involved in ischemia-reperfusion injury cascades. We aimed to study the antioxidant activities of ABM against tissue injury caused by myocardial ischemia- reperfusion processes. Methods: A rat cardiomyocyte H9c2 cell line was set up and used for the study. Cell viabilities after different dosages of ABM extract treatment and hydrogen peroxide injuries were checked. A cardiac ischemia-reperfusion injury model was set up to elucidate the cardioprotective roles of ABM extract. Different doses of 22.5 mg/kg of body weight and 45 mg/kg of body weight ABM extract were pre-treated at 24 and 48 hours before inducing the ischemia-reperfusion injury. Results: ABM extract treatment increased the survival of cardiomyocytes without cytotoxicity. Pretreatment with ABM extract reduced hydrogen peroxide-induced cell damage and increased cell survival (0.86 ± 0.15 in ABM 6.0 mg/ml vs. 0.45 ± 0.03 in control, p = 0.019). ABM-pretreated rats that underwent myocardial ischemia-reperfusion had greatly reduced infarct areas compared to those in the control group (14.3 ± 3.3% in control group, 4.6 ± 1.9% in 22.5 mg/kg group, 4.9 ± 2.1% in 45 mg/kg group, p < 0.05). Conclusion: ABM increases antioxidant activity, which greatly ameliorates myocardial injuries caused by myocardial ischemia-reperfusion injuries. Using ABM as a health food may provide cardioprotective effect against ischemia-reperfusion injuries.

UR - http://www.scopus.com/inward/record.url?scp=79952797251&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79952797251&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:79952797251

VL - 26

SP - 235

EP - 241

JO - Acta Cardiologica Sinica

JF - Acta Cardiologica Sinica

SN - 1011-6842

IS - 4

ER -

Huang CH, Tsai HN, Peng RH, Hsieh YH, Chuang W-J, Hsu GC et al. Agaricus blazei Murill ameliorates myocardial ischemia-reperfusion injury. Acta Cardiologica Sinica. 2010 Dec 1;26(4):235-241.