AH-DB: Collecting protein structure pairs before and after binding

Darby Tien Hao Chang, Tsung Ju Yao, Chen Yu Fan, Chih Yun Chiang, Yi Han Bai

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

This work presents the Apo-Holo DataBase (AH-DB, http://ahdb.ee.ncku.edu. tw/ and http://ahdb.csbb. ntu.edu.tw/), which provides corresponding pairs of protein structures before and after binding. Conformational transitions are commonly observed in various protein interactions that are involved in important biological functions. For example, copper-zinc superoxide dismutase (SOD1), which destroys free superoxide radicals in the body, undergoes a large conformational transition from an 'open' state (apo structure) to a 'closed' state (holo structure). Many studies have utilized collections of apo-holo structure pairs to investigate the conformational transitions and critical residues. However, the collection process is usually complicated, varies from study to study and produces a small-scale data set. AH-DB is designed to provide an easy and unified way to prepare such data, which is generated by identifying/mapping molecules in different Protein Data Bank (PDB) entries. Conformational transitions are identified based on a refined alignment scheme to overcome the challenge that many structures in the PDB database are only protein fragments and not complete proteins. There are 746 314 apo-holo pairs in AH-DB, which is about 30 times those in the second largest collection of similar data. AH-DB provides sophisticated interfaces for searching apo-holo structure pairs and exploring conformational transitions from apo structures to the corresponding holo structures.

Original languageEnglish
Pages (from-to)D472-D478
JournalNucleic acids research
Volume40
Issue numberD1
DOIs
Publication statusPublished - 2012 Jan 1

All Science Journal Classification (ASJC) codes

  • Genetics

Fingerprint Dive into the research topics of 'AH-DB: Collecting protein structure pairs before and after binding'. Together they form a unique fingerprint.

  • Cite this