Algorithmic robustness to preferred orientations in single particle analysis by CryoEM

C. O.S. Sorzano, D. Semchonok, S. C. Lin, Y. C. Lo, J. L. Vilas, A. Jiménez-Moreno, M. Gragera, S. Vacca, D. Maluenda, M. Martínez, E. Ramírez-Aportela, R. Melero, A. Cuervo, J. J. Conesa, P. Conesa, P. Losana, L. del Caño, J. Jiménez de la Morena, Y. C. Fonseca, R. Sánchez-GarcíaD. Strelak, E. Fernández-Giménez, F. de Isidro, D. Herreros, P. L. Kastritis, R. Marabini, B. D. Bruce, J. M. Carazo

Research output: Contribution to journalArticlepeer-review


The presence of preferred orientations in single particle analysis (SPA) by cryo-Electron Microscopy (cryoEM) is currently one of the hurdles preventing many structural analyses from yielding high-resolution structures. Although the existence of preferred orientations is mostly related to the grid preparation, in this technical note, we show that some image processing algorithms used for angular assignment and three-dimensional (3D) reconstruction are more robust than others to these detrimental conditions. We exemplify this argument with three different data sets in which the presence of preferred orientations hindered achieving a 3D reconstruction without artifacts or, even worse, a 3D reconstruction could never be achieved.

Original languageEnglish
Article number107695
JournalJournal of Structural Biology
Issue number1
Publication statusPublished - 2021 Mar

All Science Journal Classification (ASJC) codes

  • Structural Biology


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