Allergen exposure induces adipose tissue inflammation and insulin resistance

Chien Cheng Jung, Yau Sheng Tsai, Chih Ching Chang, Tsun Jen Cheng, Ching Wen Chang, Ping Yen Liu, Yi Jen Chiu, Huey Jen Su

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

This study investigates whether exposure to allergen elicits insulin resistance as a result of adipose tissue inflammation. Male C57BL/6 mice were challenged with ovalbumin (OVA) allergen for 12 weeks, and blood and adipose tissue samples were collected at 24 h after the last challenge. Levels of adhesion molecules, fasting insulin, fasting glucose, and adipokines in the blood were analyzed, and fasting homeostasis model assessment was applied to determine insulin resistance (HOMA-IR). The expression of pro- and anti-inflammatory genes in dissected adipose tissues was analyzed by real-time RT-PCR. Our results showed that OVA exposure increased insulin resistance as well as resistin and E-selectin, but reduced adiponectin in the serum. Resistin level was significantly correlated with HOMA-IR. Moreover, in adipose tissues of OVA-challenged mice, the pro-inflammatory M1 genes were more abundant while the anti-inflammatory M2 genes were less than those of PBS-treated mice. The expressional changes of both M1 and M2 genes were significantly associated with serum levels of adiponectin, resistin, and E-selectin. Hematoxylin and eosin (HE) and immunohistochemistry (IHC) stain also showed that there was more obvious inflammation in OVA-challenged mice. In conclusion, the current study suggests the relationship between allergen-elicited adipose tissue inflammation and circulating inflammatory molecules, which are possible mediators for the development of insulin resistance. Therefore, we propose that allergen exposure might be one risk factor for insulin resistance.

Original languageEnglish
Pages (from-to)104-112
Number of pages9
JournalInternational Immunopharmacology
Volume23
Issue number1
DOIs
Publication statusPublished - 2014 Aug 26

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Allergens
Ovalbumin
Insulin Resistance
Adipose Tissue
Resistin
Inflammation
Fasting
E-Selectin
Adiponectin
Genes
Anti-Inflammatory Agents
Adipokines
Hematoxylin
Eosine Yellowish-(YS)
Serum
Inbred C57BL Mouse
Real-Time Polymerase Chain Reaction
Homeostasis
Coloring Agents
Immunohistochemistry

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Pharmacology

Cite this

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abstract = "This study investigates whether exposure to allergen elicits insulin resistance as a result of adipose tissue inflammation. Male C57BL/6 mice were challenged with ovalbumin (OVA) allergen for 12 weeks, and blood and adipose tissue samples were collected at 24 h after the last challenge. Levels of adhesion molecules, fasting insulin, fasting glucose, and adipokines in the blood were analyzed, and fasting homeostasis model assessment was applied to determine insulin resistance (HOMA-IR). The expression of pro- and anti-inflammatory genes in dissected adipose tissues was analyzed by real-time RT-PCR. Our results showed that OVA exposure increased insulin resistance as well as resistin and E-selectin, but reduced adiponectin in the serum. Resistin level was significantly correlated with HOMA-IR. Moreover, in adipose tissues of OVA-challenged mice, the pro-inflammatory M1 genes were more abundant while the anti-inflammatory M2 genes were less than those of PBS-treated mice. The expressional changes of both M1 and M2 genes were significantly associated with serum levels of adiponectin, resistin, and E-selectin. Hematoxylin and eosin (HE) and immunohistochemistry (IHC) stain also showed that there was more obvious inflammation in OVA-challenged mice. In conclusion, the current study suggests the relationship between allergen-elicited adipose tissue inflammation and circulating inflammatory molecules, which are possible mediators for the development of insulin resistance. Therefore, we propose that allergen exposure might be one risk factor for insulin resistance.",
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Allergen exposure induces adipose tissue inflammation and insulin resistance. / Jung, Chien Cheng; Tsai, Yau Sheng; Chang, Chih Ching; Cheng, Tsun Jen; Chang, Ching Wen; Liu, Ping Yen; Chiu, Yi Jen; Su, Huey Jen.

In: International Immunopharmacology, Vol. 23, No. 1, 26.08.2014, p. 104-112.

Research output: Contribution to journalArticle

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AU - Chiu, Yi Jen

AU - Su, Huey Jen

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