Alteration of loperamide-induced prostate relaxation in high-fat diet-fed rats

Sheng Lung Hsu, Hsien Hui Chung, I-Hung Chen, Yat-Ching Tong

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Objective. To investigate the change of loperamide-induced prostate relaxation in rats fed with high-fat diet (HFD). Materials and Methods. Adult male Wistar rats were divided into 2 groups: (1) control rats fed with normal chow and (2) rats fed with HFD for 6 months. The prostate was removed for histology study. Isolated prostate strips were hung in organ bath and precontracted with 1 mol/L phenylephrine or 50 mmol/L KCl. The relaxation responses to loperamide 0.1 to 10 mol/L were recorded. Western blotting analyses were performed for prostate -opioid receptors (MOR) and ATP-sensitive potassium (K ATP) channel proteins: sulfonylurea receptor (SUR) and inwardly rectifying potassium channel (Kir) 6.2 subunits. Results. Body weight, prostate weight, plasma levels of glucose, insulin, triglyceride, and cholesterol, as well as systolic blood pressure, were significantly increased in the HFD rats. Histology showed prostatic hyperplasia in the HFD rat prostate. Prostatic relaxation induced by loperamide was markedly reduced in HFD when compared to the control. Protein expressions of MOR, SUR, and Kir 6.2 were decreased in HFD-fed rats. Conclusion. Loperamide-induced prostate relaxation is decreased in HFD rats due to reduced MOR and K ATP channel expressions.

Original languageEnglish
Article number517836
JournalScientific World Journal
Volume2014
DOIs
Publication statusPublished - 2014 Jan 1

Fingerprint

Loperamide
High Fat Diet
Nutrition
fat
Rats
Prostate
Fats
diet
Sulfonylurea Receptors
Histology
Adenosine Triphosphate
histology
potassium
Rat control
Inwardly Rectifying Potassium Channel
Blood Pressure
KATP Channels
protein
Blood pressure
Prostatic Hyperplasia

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Environmental Science(all)
  • Medicine(all)

Cite this

@article{1d89e125b7184b36bd0bb50844a1cea7,
title = "Alteration of loperamide-induced prostate relaxation in high-fat diet-fed rats",
abstract = "Objective. To investigate the change of loperamide-induced prostate relaxation in rats fed with high-fat diet (HFD). Materials and Methods. Adult male Wistar rats were divided into 2 groups: (1) control rats fed with normal chow and (2) rats fed with HFD for 6 months. The prostate was removed for histology study. Isolated prostate strips were hung in organ bath and precontracted with 1 mol/L phenylephrine or 50 mmol/L KCl. The relaxation responses to loperamide 0.1 to 10 mol/L were recorded. Western blotting analyses were performed for prostate -opioid receptors (MOR) and ATP-sensitive potassium (K ATP) channel proteins: sulfonylurea receptor (SUR) and inwardly rectifying potassium channel (Kir) 6.2 subunits. Results. Body weight, prostate weight, plasma levels of glucose, insulin, triglyceride, and cholesterol, as well as systolic blood pressure, were significantly increased in the HFD rats. Histology showed prostatic hyperplasia in the HFD rat prostate. Prostatic relaxation induced by loperamide was markedly reduced in HFD when compared to the control. Protein expressions of MOR, SUR, and Kir 6.2 were decreased in HFD-fed rats. Conclusion. Loperamide-induced prostate relaxation is decreased in HFD rats due to reduced MOR and K ATP channel expressions.",
author = "Hsu, {Sheng Lung} and Chung, {Hsien Hui} and I-Hung Chen and Yat-Ching Tong",
year = "2014",
month = "1",
day = "1",
doi = "10.1155/2014/517836",
language = "English",
volume = "2014",
journal = "The Scientific World Journal",
issn = "2356-6140",
publisher = "Hindawi Publishing Corporation",

}

Alteration of loperamide-induced prostate relaxation in high-fat diet-fed rats. / Hsu, Sheng Lung; Chung, Hsien Hui; Chen, I-Hung; Tong, Yat-Ching.

In: Scientific World Journal, Vol. 2014, 517836, 01.01.2014.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Alteration of loperamide-induced prostate relaxation in high-fat diet-fed rats

AU - Hsu, Sheng Lung

AU - Chung, Hsien Hui

AU - Chen, I-Hung

AU - Tong, Yat-Ching

PY - 2014/1/1

Y1 - 2014/1/1

N2 - Objective. To investigate the change of loperamide-induced prostate relaxation in rats fed with high-fat diet (HFD). Materials and Methods. Adult male Wistar rats were divided into 2 groups: (1) control rats fed with normal chow and (2) rats fed with HFD for 6 months. The prostate was removed for histology study. Isolated prostate strips were hung in organ bath and precontracted with 1 mol/L phenylephrine or 50 mmol/L KCl. The relaxation responses to loperamide 0.1 to 10 mol/L were recorded. Western blotting analyses were performed for prostate -opioid receptors (MOR) and ATP-sensitive potassium (K ATP) channel proteins: sulfonylurea receptor (SUR) and inwardly rectifying potassium channel (Kir) 6.2 subunits. Results. Body weight, prostate weight, plasma levels of glucose, insulin, triglyceride, and cholesterol, as well as systolic blood pressure, were significantly increased in the HFD rats. Histology showed prostatic hyperplasia in the HFD rat prostate. Prostatic relaxation induced by loperamide was markedly reduced in HFD when compared to the control. Protein expressions of MOR, SUR, and Kir 6.2 were decreased in HFD-fed rats. Conclusion. Loperamide-induced prostate relaxation is decreased in HFD rats due to reduced MOR and K ATP channel expressions.

AB - Objective. To investigate the change of loperamide-induced prostate relaxation in rats fed with high-fat diet (HFD). Materials and Methods. Adult male Wistar rats were divided into 2 groups: (1) control rats fed with normal chow and (2) rats fed with HFD for 6 months. The prostate was removed for histology study. Isolated prostate strips were hung in organ bath and precontracted with 1 mol/L phenylephrine or 50 mmol/L KCl. The relaxation responses to loperamide 0.1 to 10 mol/L were recorded. Western blotting analyses were performed for prostate -opioid receptors (MOR) and ATP-sensitive potassium (K ATP) channel proteins: sulfonylurea receptor (SUR) and inwardly rectifying potassium channel (Kir) 6.2 subunits. Results. Body weight, prostate weight, plasma levels of glucose, insulin, triglyceride, and cholesterol, as well as systolic blood pressure, were significantly increased in the HFD rats. Histology showed prostatic hyperplasia in the HFD rat prostate. Prostatic relaxation induced by loperamide was markedly reduced in HFD when compared to the control. Protein expressions of MOR, SUR, and Kir 6.2 were decreased in HFD-fed rats. Conclusion. Loperamide-induced prostate relaxation is decreased in HFD rats due to reduced MOR and K ATP channel expressions.

UR - http://www.scopus.com/inward/record.url?scp=84919363224&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84919363224&partnerID=8YFLogxK

U2 - 10.1155/2014/517836

DO - 10.1155/2014/517836

M3 - Article

VL - 2014

JO - The Scientific World Journal

JF - The Scientific World Journal

SN - 2356-6140

M1 - 517836

ER -