Alterations in urinary bladder M2-muscarinic receptor protein and mRNA in 2-week streptozotocin-induced diabetic rats

Yat-Ching Tong, Wan Tai Chin, Juei Tang Cheng

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

The M2 receptor (M2-mAChR) is quantitatively the dominant muscarinic subtype in animal bladders. The alterations in its protein quantity and biosynthesis during diabetic cystopathy were investigated. Three-month-old male Wistar rats were divided into two groups: (1) 2-week-old diabetics; and (2) normoglycemic control rats. Diabetes was induced by single intravenous injection of 60 mg/kg streptozotocin. The amount of M2 receptor protein in the rat bladder body tissue was measured by Western immunoblotting using monoclonal antibodies. For determination of M2 muscarinic receptor mRNA in the bladder tissue, the method of Northern blotting was employed. The results of the Western immunoblotting showed that the amount of M2-mAChR protein in the diabetic bladder was significantly increased by 40.0±6.2% when compared with the control bladder (P<0.05, n=8). The Northern blotting demonstrated a 69.3±8.5% increase of the M2-mAChR mRNA in the diabetic bladder (P<0.05, n=8). The findings of the present study demonstrated an up-regulation of M2-mAChR biosynthesis in the diabetic urinary bladder. This phenomenon could lead to increased reactivity to acetylcholine and thus results in detrusor instability. Copyright (C) 1999 Elsevier Science Ireland Ltd.

Original languageEnglish
Pages (from-to)173-176
Number of pages4
JournalNeuroscience Letters
Volume277
Issue number3
DOIs
Publication statusPublished - 1999 Dec 31

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Muscarinic M2 Receptors
Streptozocin
Urinary Bladder
Messenger RNA
Proteins
Northern Blotting
Western Blotting
Protein Biosynthesis
Intravenous Injections
Cholinergic Agents
Acetylcholine
Wistar Rats
Up-Regulation
Monoclonal Antibodies

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

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title = "Alterations in urinary bladder M2-muscarinic receptor protein and mRNA in 2-week streptozotocin-induced diabetic rats",
abstract = "The M2 receptor (M2-mAChR) is quantitatively the dominant muscarinic subtype in animal bladders. The alterations in its protein quantity and biosynthesis during diabetic cystopathy were investigated. Three-month-old male Wistar rats were divided into two groups: (1) 2-week-old diabetics; and (2) normoglycemic control rats. Diabetes was induced by single intravenous injection of 60 mg/kg streptozotocin. The amount of M2 receptor protein in the rat bladder body tissue was measured by Western immunoblotting using monoclonal antibodies. For determination of M2 muscarinic receptor mRNA in the bladder tissue, the method of Northern blotting was employed. The results of the Western immunoblotting showed that the amount of M2-mAChR protein in the diabetic bladder was significantly increased by 40.0±6.2{\%} when compared with the control bladder (P<0.05, n=8). The Northern blotting demonstrated a 69.3±8.5{\%} increase of the M2-mAChR mRNA in the diabetic bladder (P<0.05, n=8). The findings of the present study demonstrated an up-regulation of M2-mAChR biosynthesis in the diabetic urinary bladder. This phenomenon could lead to increased reactivity to acetylcholine and thus results in detrusor instability. Copyright (C) 1999 Elsevier Science Ireland Ltd.",
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Alterations in urinary bladder M2-muscarinic receptor protein and mRNA in 2-week streptozotocin-induced diabetic rats. / Tong, Yat-Ching; Chin, Wan Tai; Cheng, Juei Tang.

In: Neuroscience Letters, Vol. 277, No. 3, 31.12.1999, p. 173-176.

Research output: Contribution to journalArticle

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