TY - JOUR
T1 - Alterations of nerve-growth factor and p75NTR expressions in urinary bladder of fructose-fed obese rats
AU - Cheng, Juei Tang
AU - Tong, Yat Ching
N1 - Funding Information:
This study was supported by grants from the National Science Council of the Republic of China (Taiwan): NSC 94-2314-B-006-086; NSC 95-2314-B-006-069; NSC 96-2314-B-017-MY3.
PY - 2008/8/15
Y1 - 2008/8/15
N2 - The study investigated the effects of metabolic syndrome on urinary bladder nerve-growth factor (NGF) and p75NTR expression in fructose-fed obese rats. Age-matched male Wistar rats were divided into four groups; group I: normal control rats; group II: 6-week fructose-fed rats (FR); group III: 9-week FR; and group IV: 12-week FR. In vivo cystometry under anesthesia was performed. In vitro bladder NGF levels were measured by enzyme-linked immunosorbent assay (ELISA). Expressions of the mRNAs that encoded NGF and NGF receptor p75NTR in control and 9-week FR bladder were studied using the method of reverse transcription combined with polymerase chain reaction (RT-PCR).The three groups of FR showed significant increases in body weight, blood pressure, plasma glucose, insulin and triglyceride levels when compared to control rats. The bladder NGF levels in the 9-week and 12-week FR were significantly lower than the control (66.8 ± 5.4, 49.4 ± 7.1 and 95.2 ± 6.5 pg/μg protein; p < 0.05 and p < 0.01 respectively, for n = 8 in each group). The bladder emptying function was deteriorated in these two groups of FR as shown by the significantly increased residual volume and decreased emptying fraction. The mRNA expressions of bladder NGF and p75NTR in the 9-week FR were significantly decreased when compared to the control group (p < 0.05 and p < 0.001 respectively, n = 8 in each group). In conclusion, long-term metabolic syndrome in FR significantly decreases bladder NGF and p75NTR expression. These alterations are associated with deterioration in bladder emptying function.
AB - The study investigated the effects of metabolic syndrome on urinary bladder nerve-growth factor (NGF) and p75NTR expression in fructose-fed obese rats. Age-matched male Wistar rats were divided into four groups; group I: normal control rats; group II: 6-week fructose-fed rats (FR); group III: 9-week FR; and group IV: 12-week FR. In vivo cystometry under anesthesia was performed. In vitro bladder NGF levels were measured by enzyme-linked immunosorbent assay (ELISA). Expressions of the mRNAs that encoded NGF and NGF receptor p75NTR in control and 9-week FR bladder were studied using the method of reverse transcription combined with polymerase chain reaction (RT-PCR).The three groups of FR showed significant increases in body weight, blood pressure, plasma glucose, insulin and triglyceride levels when compared to control rats. The bladder NGF levels in the 9-week and 12-week FR were significantly lower than the control (66.8 ± 5.4, 49.4 ± 7.1 and 95.2 ± 6.5 pg/μg protein; p < 0.05 and p < 0.01 respectively, for n = 8 in each group). The bladder emptying function was deteriorated in these two groups of FR as shown by the significantly increased residual volume and decreased emptying fraction. The mRNA expressions of bladder NGF and p75NTR in the 9-week FR were significantly decreased when compared to the control group (p < 0.05 and p < 0.001 respectively, n = 8 in each group). In conclusion, long-term metabolic syndrome in FR significantly decreases bladder NGF and p75NTR expression. These alterations are associated with deterioration in bladder emptying function.
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U2 - 10.1016/j.neulet.2008.06.008
DO - 10.1016/j.neulet.2008.06.008
M3 - Article
C2 - 18585860
AN - SCOPUS:46149115119
SN - 0304-3940
VL - 441
SP - 25
EP - 28
JO - Neuroscience Letters
JF - Neuroscience Letters
IS - 1
ER -