Amotl2 is essential for cell movements in zebrafish embryo and regulates c-Src translocation

Huizhe Huang, Fu I. Lu, Shunji Jia, Shu Meng, Ying Cao, Yeqi Wang, Weiping Ma, Kun Yin, Zilong Wen, Jingrong Peng, Christine Thisse, Bernard Thisse, Anming Meng

Research output: Contribution to journalArticlepeer-review

43 Citations (Scopus)

Abstract

Angiomotin (Amot), the founding member of the Motin family, is involved in angiogenesis by regulating endothelial cell motility, and is required for visceral endoderm movement in mice. However, little is known about biological functions of the other two members of the Motin family, Angiomotin-like1 (Amotl1) and Angiomotin-like2 (Amotl2). Here, we have identified zebrafish amotl2 as an Fgf-responsive gene. Zebrafish amotl2 is expressed maternally and in restricted cell types zygotically. Knockdown of amotl2 expression delays epiboly and impairs convergence and extension movement, and amotl2-deficient cells in mosaic embryos fail to migrate properly. This coincides with loss of membrane protrusions and disorder of F-actin. Amotl2 partially co-localizes with RhoB- or EEA1-positive endosomes and the non-receptor tyrosine kinase c-Src. We further demonstrate that Amotl2 interacts preferentially with and facilitates outward translocation of the phosphorylated c-Src, which may in turn regulate the membrane architecture. These data provide the first evidence that amotl2 is essential for cell movements in vertebrate embryos.

Original languageEnglish
Pages (from-to)979-988
Number of pages10
JournalDevelopment
Volume134
Issue number5
DOIs
Publication statusPublished - 2007 Mar

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Developmental Biology

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