TY - JOUR
T1 - Amphiregulin as a tumor promoter for oral squamous cell carcinoma
T2 - Involvement of cyclooxygenase 2
AU - Tsai, Sen Tien
AU - Yang, Kai Ying
AU - Jin, Ying Tai
AU - Lin, Yen Chun
AU - Chang, Mei Tzu
AU - Wu, Li Wha
N1 - Funding Information:
This manuscript was supported by the National Science Council in Taiwan (NSC91-2314-B-006-053 and NSC-93-2314-B006-041).
PY - 2006/4
Y1 - 2006/4
N2 - Amphiregulin (AR), an epidermal growth factor (EGF)-like molecule, is a mitogen for keratinocytes. Squamous cell carcinoma (SCC) is a tumor derived from keratinoctyes. Expression of AR mRNA positively correlated with the clinical progression of 39 oral SCC. Oral SCC line, KB, was used as a model to study if increased expression of AR altered the biological behaviors of SCC cells. Exogenous AR dose-dependently enhanced the proliferation of KB cells expressing EGF receptor 1 (EGFR-1), a major receptor for AR, but little AR. Neutralizing anti-AR antibody significantly reversed the stimulatory effect of exogenous AR on KB cell proliferation. Ectopically expressed AR in stable clones manifested higher abilities to proliferate, migrate and invade Matrigel than vector control. Cyclooxygenase 2 (COX-2), but not metalloprotease 9 (MMP-9) mRNA, was increased in all the AR-expressing stable clones. In summary, AR behaves as a tumor promoter for oral SCC cells partly via increased expression of COX-2.
AB - Amphiregulin (AR), an epidermal growth factor (EGF)-like molecule, is a mitogen for keratinocytes. Squamous cell carcinoma (SCC) is a tumor derived from keratinoctyes. Expression of AR mRNA positively correlated with the clinical progression of 39 oral SCC. Oral SCC line, KB, was used as a model to study if increased expression of AR altered the biological behaviors of SCC cells. Exogenous AR dose-dependently enhanced the proliferation of KB cells expressing EGF receptor 1 (EGFR-1), a major receptor for AR, but little AR. Neutralizing anti-AR antibody significantly reversed the stimulatory effect of exogenous AR on KB cell proliferation. Ectopically expressed AR in stable clones manifested higher abilities to proliferate, migrate and invade Matrigel than vector control. Cyclooxygenase 2 (COX-2), but not metalloprotease 9 (MMP-9) mRNA, was increased in all the AR-expressing stable clones. In summary, AR behaves as a tumor promoter for oral SCC cells partly via increased expression of COX-2.
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U2 - 10.1016/j.oraloncology.2005.09.005
DO - 10.1016/j.oraloncology.2005.09.005
M3 - Article
C2 - 16376136
AN - SCOPUS:33645093975
SN - 1368-8375
VL - 42
SP - 381
EP - 390
JO - Oral Oncology
JF - Oral Oncology
IS - 4
ER -