TY - JOUR
T1 - An AC electrokinetics-based electrochemical aptasensor for the rapid detection of microRNA-155
AU - Ondevilla, Neil Adrian P.
AU - Wong, Tak Wah
AU - Lee, Nan Yao
AU - Chang, Hsien Chang
N1 - Funding Information:
This research was supported by the Ministry of Science and Technology, R.O.C. (MOST 109-2221-E-006-035 ). The fabrication of ACEO electrodes was done with the help of the National Nano Device Laboratories (NDL) Taiwan by supplying the micro-fabrication equipment. Additional electrodes were fabricated by the team of Prof. Jian-Jang Huang from the Graduate Institute of Photonics and Optoelectronics, National Taiwan University. Dr. T.W. Wong acknowledges funding from MOST 109-2327-B-006-005 , and the Center of Applied Nanomedicine, National Cheng Kung University from the Featured Areas Research Center Program within the framework of the Higher Education Sprout Project by the Ministry of Education (MOE) in Taiwan.
Funding Information:
This research was supported by the Ministry of Science and Technology, R.O.C. (MOST 109-2221-E-006-035). The fabrication of ACEO electrodes was done with the help of the National Nano Device Laboratories (NDL) Taiwan by supplying the micro-fabrication equipment. Additional electrodes were fabricated by the team of Prof. Jian-Jang Huang from the Graduate Institute of Photonics and Optoelectronics, National Taiwan University. Dr. T.W. Wong acknowledges funding from MOST 109-2327-B-006-005, and the Center of Applied Nanomedicine, National Cheng Kung University from the Featured Areas Research Center Program within the framework of the Higher Education Sprout Project by the Ministry of Education (MOE) in Taiwan.
Publisher Copyright:
© 2021 Elsevier B.V.
PY - 2022/3/1
Y1 - 2022/3/1
N2 - Traditional immunosensors are often limited by low sensitivity and long detection times, for they usually depend on passive diffusion-dominated transport of target analytes for the binding reaction with a bio-recognition element such as enzymes, antibodies, and aptamers. Numerous studies rely on electric field manipulation by using alternating current (AC) electrokinetics to enhance the hybridization rate and reduce the hybridization time for faster and more efficient detection. This study demonstrated a rapid electrochemical aptasensor integrated with an AC electroosmotic (ACEO) flow phenomenon for the enhanced target hybridization of microRNA-155 (miR-155). Optimization of the electrokinetic conditions for target collection resulted in a saturation point after 75 s miR-155 was detected within the range of 1 aM–10 pM with a detection limit of 1 aM, which is 100 times lower and about 50 times faster compared with the conventional diffusion-dependent detection done for 1 h. The detection was also done in spiked serum samples, and a concentration range within the required detection range was obtained. The highly sensitive and specific results allow for the rapid and real-time sensing of target biomarkers, which can be used for the early detection of infection.
AB - Traditional immunosensors are often limited by low sensitivity and long detection times, for they usually depend on passive diffusion-dominated transport of target analytes for the binding reaction with a bio-recognition element such as enzymes, antibodies, and aptamers. Numerous studies rely on electric field manipulation by using alternating current (AC) electrokinetics to enhance the hybridization rate and reduce the hybridization time for faster and more efficient detection. This study demonstrated a rapid electrochemical aptasensor integrated with an AC electroosmotic (ACEO) flow phenomenon for the enhanced target hybridization of microRNA-155 (miR-155). Optimization of the electrokinetic conditions for target collection resulted in a saturation point after 75 s miR-155 was detected within the range of 1 aM–10 pM with a detection limit of 1 aM, which is 100 times lower and about 50 times faster compared with the conventional diffusion-dependent detection done for 1 h. The detection was also done in spiked serum samples, and a concentration range within the required detection range was obtained. The highly sensitive and specific results allow for the rapid and real-time sensing of target biomarkers, which can be used for the early detection of infection.
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U2 - 10.1016/j.bios.2021.113847
DO - 10.1016/j.bios.2021.113847
M3 - Article
C2 - 34902642
AN - SCOPUS:85120900207
SN - 0956-5663
VL - 199
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
M1 - 113847
ER -