TY - JOUR
T1 - An alternative method to quantify 2-MIB producing cyanobacteria in drinking water reservoirs
T2 - Method development and field applications
AU - Chiu, Yi Ting
AU - Yen, Hung Kai
AU - Lin, Tsair Fuh
N1 - Funding Information:
This study is funded in part by the projects of Taiwan Ministry of Science and Technology ( NSC 102-2119-M-002-008 , MOST 103-2119-M-002-004 , and MOST 104-2119-M-002-001 ), and in part by the projects of Water Resources Agency, Taiwan Ministry of Economic Affairs ( MOEAWRA1020152 , MOEAWRA1030051 , and MOEAWRA1040257 ).
Publisher Copyright:
© 2016 Elsevier Inc.
PY - 2016/11/1
Y1 - 2016/11/1
N2 - 2-Methylisoborneol (2-MIB) is a commonly detected cyanobacterial odorant in drinking water sources in many countries. To provide safe and high-quality water, development of a monitoring method for the detection of 2-MIB-synthesis (mibC) genes is very important. In this study, new primers MIBS02F/R intended specifically for the mibC gene were developed and tested. Experimental results show that the MIBS02F/R primer set was able to capture 13 2-MIB producing cyanobacterial strains grown in the laboratory, and to effectively amplify the targeted DNA region from 17 2-MIB-producing cyanobacterial strains listed in the literature. The primers were further coupled with a TaqMan probe to detect 2-MIB producers in 29 drinking water reservoirs (DWRs). The results showed statistically significant correlations between mibC genes and 2-MIB concentrations for the data from each reservoir (R2=0.413–0.998; p<0.05), from all reservoirs in each of the three islands (R2=0.302–0.796; p<0.01), and from all data of the three islands (R2=0.473–0.479; p<0.01). The results demonstrate that the real-time PCR can be an alternative method to provide information to managers of reservoirs and water utilities facing 2-MIB-related incidents.
AB - 2-Methylisoborneol (2-MIB) is a commonly detected cyanobacterial odorant in drinking water sources in many countries. To provide safe and high-quality water, development of a monitoring method for the detection of 2-MIB-synthesis (mibC) genes is very important. In this study, new primers MIBS02F/R intended specifically for the mibC gene were developed and tested. Experimental results show that the MIBS02F/R primer set was able to capture 13 2-MIB producing cyanobacterial strains grown in the laboratory, and to effectively amplify the targeted DNA region from 17 2-MIB-producing cyanobacterial strains listed in the literature. The primers were further coupled with a TaqMan probe to detect 2-MIB producers in 29 drinking water reservoirs (DWRs). The results showed statistically significant correlations between mibC genes and 2-MIB concentrations for the data from each reservoir (R2=0.413–0.998; p<0.05), from all reservoirs in each of the three islands (R2=0.302–0.796; p<0.01), and from all data of the three islands (R2=0.473–0.479; p<0.01). The results demonstrate that the real-time PCR can be an alternative method to provide information to managers of reservoirs and water utilities facing 2-MIB-related incidents.
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U2 - 10.1016/j.envres.2016.08.034
DO - 10.1016/j.envres.2016.08.034
M3 - Article
C2 - 27607443
AN - SCOPUS:84984907209
SN - 0013-9351
VL - 151
SP - 618
EP - 627
JO - Environmental Research
JF - Environmental Research
ER -
