An Aptamer Based Sandwich Assay for Simultaneous Detection of Multiple Cardiovascular Biomarkers on A Multilayered Integrated Microfluidic System

Anirban Sinha, Priya Gopinathan, Yi Da Chung, Shu-Chu Shiesh, Gwo Bin Lee

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Cardiovascular diseases (CVDs) have resulted in an estimated death toll of nearly 17 million yearly. Early detection of CVDs may reduce the risk of sudden deaths. The current technologies based on antigen-antibody interactions have limitations in point-of-care (POC) applications. This study therefore reports an aptamer magnetic bead-based assay which is relatively sensitive and highly selective on a new integrated microfluidic platform for simultaneous analysis of up to six untreated clinical serum samples utilizing less than a volume of 10 μL from each patient. Experimental results showed that one of the important biomarkers for CVDs, cardiac troponin I, could be detected on the microfluidic platform in less than 35 minutes with a limit of detection of 0.01 ng/mL. It may provide a promising device for POC applications.

Original languageEnglish
Title of host publication2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages1075-1077
Number of pages3
ISBN (Electronic)9781728120072
DOIs
Publication statusPublished - 2019 Jun 1
Event20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII - Berlin, Germany
Duration: 2019 Jun 232019 Jun 27

Publication series

Name2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII

Conference

Conference20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII
CountryGermany
CityBerlin
Period19-06-2319-06-27

Fingerprint

biomarkers
Sandwich
Microfluidics
Biomarkers
Assays
death
platforms
Sudden Death
Troponin I
antigens
Antigens
antibodies
Antibody
Antibodies
beads
Cardiac
serums
Experimental Results
Interaction
interactions

All Science Journal Classification (ASJC) codes

  • Process Chemistry and Technology
  • Spectroscopy
  • Electrical and Electronic Engineering
  • Mechanical Engineering
  • Electronic, Optical and Magnetic Materials
  • Control and Optimization
  • Instrumentation

Cite this

Sinha, A., Gopinathan, P., Chung, Y. D., Shiesh, S-C., & Lee, G. B. (2019). An Aptamer Based Sandwich Assay for Simultaneous Detection of Multiple Cardiovascular Biomarkers on A Multilayered Integrated Microfluidic System. In 2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII (pp. 1075-1077). [8808551] (2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/TRANSDUCERS.2019.8808551
Sinha, Anirban ; Gopinathan, Priya ; Chung, Yi Da ; Shiesh, Shu-Chu ; Lee, Gwo Bin. / An Aptamer Based Sandwich Assay for Simultaneous Detection of Multiple Cardiovascular Biomarkers on A Multilayered Integrated Microfluidic System. 2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII. Institute of Electrical and Electronics Engineers Inc., 2019. pp. 1075-1077 (2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII).
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Sinha, A, Gopinathan, P, Chung, YD, Shiesh, S-C & Lee, GB 2019, An Aptamer Based Sandwich Assay for Simultaneous Detection of Multiple Cardiovascular Biomarkers on A Multilayered Integrated Microfluidic System. in 2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII., 8808551, 2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII, Institute of Electrical and Electronics Engineers Inc., pp. 1075-1077, 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII, Berlin, Germany, 19-06-23. https://doi.org/10.1109/TRANSDUCERS.2019.8808551

An Aptamer Based Sandwich Assay for Simultaneous Detection of Multiple Cardiovascular Biomarkers on A Multilayered Integrated Microfluidic System. / Sinha, Anirban; Gopinathan, Priya; Chung, Yi Da; Shiesh, Shu-Chu; Lee, Gwo Bin.

2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII. Institute of Electrical and Electronics Engineers Inc., 2019. p. 1075-1077 8808551 (2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Sinha A, Gopinathan P, Chung YD, Shiesh S-C, Lee GB. An Aptamer Based Sandwich Assay for Simultaneous Detection of Multiple Cardiovascular Biomarkers on A Multilayered Integrated Microfluidic System. In 2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII. Institute of Electrical and Electronics Engineers Inc. 2019. p. 1075-1077. 8808551. (2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII). https://doi.org/10.1109/TRANSDUCERS.2019.8808551